Rabbit HDAC1 Polyclonal Antibody | anti-HDAC1 antibody

HDAC1 Polyclonal Antibody

Cat. Num. AAA10632

• Gene Names: HDAC1; HD1; RPD3; GON-10; RPD3L1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: HDAC1, Antibody; HDAC1 Polyclonal Antibody; HDAC1; DKFZp686H12203; GON-10; HD1; RPD3; RPD3L1; anti-HDAC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 482

• Applicable Applications for anti-HDAC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200; IP: 1:50 - 1:200; ChIP: 1:20 - 1:100
• Species: Human
• Route: Synthetic Peptide
• Immunogen: N term -peptide of human HDAC1
• Calculated Molecular Weight: 55kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cells using HDAC1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human esophagus using HDAC1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human lung cancer using HDAC1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using HDAC1 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-HDAC1 antibody

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

Product Categories/Family for anti-HDAC1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 2498443
• NCBI GeneID: 3065
• UniProt Accession #: Q13547; Q92534
• Molecular Weight: 482
• NCBI Official Full Name: Histone deacetylase 1
• NCBI Official Synonym Full Names: histone deacetylase 1
• NCBI Official Symbol: HDAC1
• NCBI Official Synonym Symbols: HD1; RPD3; GON-10; RPD3L1
• NCBI Protein Information: histone deacetylase 1; reduced potassium dependency, yeast homolog-like 1
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: HDAC1
• UniProt Synonym Gene Names: RPD3L1; HD1
• UniProt Entry Name: HDAC1_HUMAN