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WB (Western Blot)
(HES1 antibody - N-terminal region validated by WB using transfected Ls174Tcell lysate at 1ug/ml.)
Rabbit HES1 Polyclonal Antibody | anti-HES1 antibody
HES1 antibody - N-terminal region
Gene Names
HES1; HHL; HRY; HES-1; bHLHb39
Reactivity
Tested Species Reactivity: Human MousePredicted Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
Applications
Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A purified
Synonyms
HES1, Antibody; HES1 antibody - N-terminal region; anti-HES1 antibody
Host
Rabbit
Reactivity
Tested Species Reactivity: Human Mouse
Predicted Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
Predicted Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
Clonality
Polyclonal
Purity/Purification
Protein A purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
1.0 mg/mL (varies by lot)
Sequence
Synthetic peptide located within the following region: NTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSR
Sequence Length
280
Applicable Applications for anti-HES1 antibody
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Protein Size (#AA)
280 amino acids
Homology
Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human HES1
Protein Interactions
STAT3; JAK2; APH1A; LTBR; ASGR2; TLE1; ELAVL1; PARP1; UBC; NUDT3; HDAC6; YWHAB; PTK2; NR4A1; HMGCL; GAPDH; FHL1; CSNK1E; APCS; FANCE; FANCC; FANCA; FANCL; SPTAN1; HSP90AA1; HSPA4; FANCG; FANCF; HDAC1; RUNX2; HES6; ID4; ID1; SIRT1; UBQLN1; HEY2; ID2; ID3;
Sample Type Confirmation
HES1 is strongly supported by BioGPS gene expression data to be expressed in Raji
Preparation and Storage
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(HES1 antibody - N-terminal region validated by WB using transfected Ls174Tcell lysate at 1ug/ml.)
WB (Western Blot)
(HES1 antibody - N-terminal region validated by WB using transfected Ls174Tcell lysate at 1ug/ml.)
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 30 kDa isoform is identified, and a second isoform of 23 kDa is also present in some samples in N36.)
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 30 kDa isoform is identified, and a second isoform of 23 kDa is also present in some samples in N36.)
WB (Western Blot)
(WB Suggested Anti-HES1 Antibody Titration: 1.25ug/mlPositive Control: Raji cell lysateHES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells)
WB (Western Blot)
(WB Suggested Anti-HES1 Antibody Titration: 1.25ug/mlPositive Control: Raji cell lysateHES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells)
WB (Western Blot)
(HES1 antibody - N-terminal region validated by WB using Mouse embryo tissue at 1:800.)
WB (Western Blot)
(HES1 antibody - N-terminal region validated by WB using Mouse embryo tissue at 1:800.)
IP (Immunoprecipitation)
(K562)
IP (Immunoprecipitation)
(K562)
IHC (Immunohistochemistry)
(Rabbit Anti-HES1 AntibodyParaffin Embedded Tissue: Human cardiac cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)
IHC (Immunohistochemistry)
(Rabbit Anti-HES1 AntibodyParaffin Embedded Tissue: Human cardiac cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)
IF (Immunofluorescence)
(transfected Ls174T cell lysate at 50ug/ml.)
IF (Immunofluorescence)
(transfected Ls174T cell lysate at 50ug/ml.)
Related Product Information for anti-HES1 antibody
This is a rabbit polyclonal antibody against HES1. It was validated on Western Blot and immunohistochemistry
Target Description: HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Target Description: HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Product Categories/Family for anti-HES1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30kDa
NCBI Official Full Name
transcription factor HES-1
NCBI Official Synonym Full Names
hes family bHLH transcription factor 1
NCBI Official Symbol
HES1
NCBI Official Synonym Symbols
HHL; HRY; HES-1; bHLHb39
NCBI Protein Information
transcription factor HES-1
UniProt Protein Name
Transcription factor HES-1
UniProt Gene Name
HES1
UniProt Synonym Gene Names
BHLHB39; HL; HRY; bHLHb39; hHL
UniProt Entry Name
HES1_HUMAN
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Product Notes
The HES1 hes1 (Catalog #AAA23407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HES1 antibody - N-terminal region reacts with Tested Species Reactivity: Human Mouse Predicted Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's HES1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the HES1 hes1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: NTTPDKPKTA SEHRKSSKPI MEKRRRARIN ESLSQLKTLI LDALKKDSSR. It is sometimes possible for the material contained within the vial of "HES1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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