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IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U-2 OS cells using Histone H2AX Polyclonal Antibody.)
Rabbit Histone H2AX Polyclonal Antibody | anti-H2AX antibody
Histone H2AX Rabbit Polyclonal Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
Histone H2AX, Antibody; Histone H2AX Rabbit Polyclonal Antibody; H2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; histone H2AX; gamma H2A.X; ??H2AX; anti-H2AX antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-H2AX antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
A synthetic peptide of human Histone H2AX (NP_002096.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U-2 OS cells using Histone H2AX Polyclonal Antibody.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U-2 OS cells using Histone H2AX Polyclonal Antibody.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of HeLa cells using Histone H2AX Polyclonal Antibody.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of HeLa cells using Histone H2AX Polyclonal Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using Histone H2AX antibody.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using Histone H2AX antibody.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using Histone H2AX antibody.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using Histone H2AX antibody.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat testis using Histone H2AX antibody.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat testis using Histone H2AX antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Histone H2AX antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Histone H2AX antibody.)
Related Product Information for anti-H2AX antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Product Categories/Family for anti-H2AX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,145 Da
NCBI Official Full Name
histone H2A.x
NCBI Official Synonym Full Names
H2A histone family, member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2A.x; H2AX histone
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
Similar Products
Product Notes
The H2AX h2afx (Catalog #AAA30721) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2AX Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2AX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the H2AX h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2AX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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