Application Data
(Shen, Tong, et al. "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." Journal of hematology & oncology 11.1 (2018): 95.)
Rabbit Histone H4 Polyclonal Antibody | anti-H4 antibody
Histone H4 (Acetyl-Lys16) Rabbit Polyclonal Antibody
WB: 1:1000-2000
IHC: 1:50-300
Application Data
(Shen, Tong, et al. "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." Journal of hematology & oncology 11.1 (2018): 95.)
Application Data
(Shen, Tong, et al. "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." Journal of hematology & oncology 11.1 (2018): 95.)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)
NCBI and Uniprot Product Information
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Product Notes
The H4 hist1h4a (Catalog #AAA30783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H4 (Acetyl-Lys16) Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P),. IF: 1:50-200 WB: 1:1000-2000 IHC: 1:50-300. Researchers should empirically determine the suitability of the H4 hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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