Rabbit HNRNPH3 Polyclonal Antibody | anti-HNRNPH3 antibody

Anti-HNRNPH3 Antibody

Cat. Num. AAA19338

• Gene Names: HNRNPH3; 2H9; HNRPH3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: HNRNPH3, Antibody; Anti-HNRNPH3 Antibody; HNRNPH3; HNRPH3; Heterogeneous nuclear ribonucleoprotein H3; hnRNP H3; Heterogeneous nuclear ribonucleoprotein 2H9; hnRNP 2H9; heterogeneous nuclear ribonucleoprotein H3; anti-HNRNPH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for HNRNPH3 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-HNRNPH3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Human, Mouse, Rat|; IHC-P: 1-2ug/ml|Human, Mouse, Rat|; ICC/IF: 5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human HNRNPH3 recombinant protein (Position: K29-N268).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 10. Flow Cytometry analysis of K562 cells using anti-HNRNPH3 antibody (AAA19338).Overlay histogram showing K562 cells stained with AAA19338 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH3 Antibody (AAA19338, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 9. IF analysis of HNRNPH3 using anti- HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry)

(Figure 8. IHC analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 7. IHC analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry)

(Figure 6. IHC analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in paraffin-embedded section of human gastric cancer lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).HNRNPH3 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HNRNPH3 Antibody (AAA19338) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of HNRNPH3 using anti-HNRNPH3 antibody (AAA19338).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: human K562 whole cell lysatesLane 5: human Caco-2 whole cell lysatesLane 6: human MCF-7 whole cell lysatesLane 7: human HL-60 whole cell lysatesLane 8: human PC-3 whole cell lysatesLane 9: rat brain tissue lysatesLane 10: rat PC-12 whole cell lysatesLane 11: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HNRNPH3 antigen affinity purified polyclonal antibody (Catalog # AAA19338) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for HNRNPH3 at approximately 35KD, 37KD, 50KD. The expected band size for HNRNPH3 is at 35KD, 37KD, 50KD.)

Related Product Information for anti-HNRNPH3 antibody

Heterogeneous nuclear ribonucleoprotein H3 is a protein that in humans is encoded by the HNRNPH3 gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is localized in nuclear bodies of the nucleus. This protein is involved in the splicing process and it also participates in early heat shock-induced splicing arrest by transiently leaving the hnRNP complexes. Several alternatively spliced transcript variants have been noted for this gene, however, not all are fully characterized.

References

1. Mahe, D., Mahl, P., Gattoni, R., Fischer, N., Mattei, M. -G., Stevenin, J., Fuchs, J. -P. Cloning of human 2H9 heterogeneous nuclear ribonucleoproteins. J. Biol. Chem. 272: 1827-1836, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 1016841164
• NCBI GeneID: 3189
• NCBI Accession #: NP_001309363.1
• NCBI GenBank Nucleotide #: NM_001322434.1
• UniProt Accession #: P31942; Q9BSX1; Q9NP53; Q9NP96; Q9NPA7; Q9NPI4; Q9UFU4; Q9Y4J5; A8K682; B3KRE1
• Molecular Weight: 15,413 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein H3 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein H3
• NCBI Official Symbol: HNRNPH3
• NCBI Official Synonym Symbols: 2H9; HNRPH3
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein H3
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein H3
• UniProt Gene Name: HNRNPH3
• UniProt Synonym Gene Names: HNRPH3; hnRNP H3; hnRNP 2H9