Rabbit anti-Human, Mouse ICLN Polyclonal Antibody | anti-ICLN antibody

Rabbit anti-ICLN Antibody, Affinity Purified

Cat. Num. AAA23778

• Gene Names: CLNS1A; CLCI; ICln; CLNS1B
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Antigen Affinity Purified
• Synonyms: ICLN, Antibody; Rabbit anti-ICLN Antibody, Affinity Purified; CLNS1B; ICln; reticulocyte protein ICln; methylosome subunit pICln; i(Cln); chloride ion current inducer protein; chloride channel regulatory protein; chloride conductance regulatory protein ICln; reticulocyte pICln; chloride channel, nucleotide sensitive 1A; CLCI; Methylosome subunit pICln; chloride nucleotide-sensitive channel 1A; anti-ICLN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-ICLN antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:2000-1:10,000; IP: 6ug/mg lysate; IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
• Predicted Reactivity: Rat, Dog, Rabbit, Orangutan
• Immunogen: Between 187 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse ICLN by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human ICLN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for IP at 6 ug per reaction. ICLN was also immunoprecipitated by rabbit anti-ICLN antibody BL17109. For blotting immunoprecipitated ICLN, AAA23778 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemistry)

(Detection of human ICLN by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. AAA23778 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of mouse ICLN by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. AAA23778 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

FCM (Flow Cytometry)

(Detection of human ICLN (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-ICLN polyclonal antibody (AAA23778) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of mouse ICLN (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-ICLN polyclonal antibody (AAA23778) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for anti-ICLN antibody

Chloride channel nucleotide sensitive 1A (ICLN) is a protein that functions in multiple regulatory pathways. ICLN complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization.ICLN is also found associated with the plasma membrane where it functions as a chloride current regulator [taken from NCBI Entrez Gene (Gene ID: 1207)].

Product Categories/Family for anti-ICLN antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502891
• NCBI GeneID: 1207
• NCBI Accession #: NP_001284.1
• NCBI GenBank Nucleotide #: NM_001293.2
• UniProt Accession #: P54105; Q0VDK6; Q9NRD2; Q9NRD3; B2RCS9
• Molecular Weight: 26,215 Da
• NCBI Official Full Name: methylosome subunit pICln
• NCBI Official Synonym Full Names: chloride channel, nucleotide-sensitive, 1A
• NCBI Official Symbol: CLNS1A
• NCBI Official Synonym Symbols: CLCI; ICln; CLNS1B
• NCBI Protein Information: methylosome subunit pICln; i(Cln); reticulocyte pICln; reticulocyte protein ICln; chloride channel regulatory protein; chloride ion current inducer protein; chloride channel, nucleotide sensitive 1A; chloride conductance regulatory protein ICln
• UniProt Protein Name: Methylosome subunit pICln
• UniProt Gene Name: CLNS1A
• UniProt Synonym Gene Names: CLCI; ICLN; I(Cln); ClCI
• UniProt Entry Name: ICLN_HUMAN