Rabbit JAK3 Polyclonal Antibody | anti-JAK3 antibody

Phospho-JAK3 (Tyr904) Antibody

Cat. Num. AAA31404

• Gene Names: JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: JAK3, Antibody; Phospho-JAK3 (Tyr904) Antibody; EC 2.7.10.2; JAK 3; JAK L; JAK-3; Jak3; JAK3 HUMAN; JAK3_HUMAN; JAKL; Janus kinase 3 (a protein tyrosine kinase, leukocyte); Janus kinase 3; Janus Kinase3; L JAK; L-JAK; Leukocyte janus kinase; LJAK; Protein tyrosine kinase leukocyte; Tyrosine protein kinase JAK3; Tyrosine-protein kinase JAK3; anti-JAK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-JAK3 (Tyr904) Antibody detects endogenous levels of JAK3 only when phosphorylated at Tyr904.; Tissue Specificity: In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-JAK3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human JAK3 around the phosphorylation site of Tyr904.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).
• Subunit Structure: Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.
• Similarity: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
• Subcellular Location: Endomembrane system>Peripheral membrane protein. Cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Application Data

(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry)

(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of JAK3 (Phospho-Tyr904) using SW626 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot)

(Western blot analysis of extracts from various samples, using Phospho-JAK3 (Tyr904) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: HepG2 cells(heat shock treatment).)

Related Product Information for anti-JAK3 antibody

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

NCBI and Uniprot Product Information

• NCBI GI #: 47157315
• NCBI GeneID: 3718
• NCBI Accession #: NP_000206.2
• NCBI GenBank Nucleotide #: NM_000215.3
• UniProt Accession #: P52333; Q13259; Q13260; Q13611; Q99699; Q9Y6S2
• Molecular Weight: 125,099 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK3
• NCBI Official Synonym Full Names: Janus kinase 3
• NCBI Official Symbol: JAK3
• NCBI Official Synonym Symbols: JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• NCBI Protein Information: tyrosine-protein kinase JAK3; leukocyte Janus kinase; Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• UniProt Protein Name: Tyrosine-protein kinase JAK3
• UniProt Gene Name: JAK3
• UniProt Synonym Gene Names: JAK-3; L-JAK
• UniProt Entry Name: JAK3_HUMAN