Rabbit anti-Human, mouse LTF Polyclonal Antibody | anti-LTF antibody

LTF Antibody

Cat. Num. AAA28680

• Gene Names: LTF; LF; HLF2; GIG12; HEL110
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: LTF, Antibody; LTF Antibody; Lactotransferrin; Lactoferrin; 3421-; Growth-inhibiting protein 12; Talalactoferrin; Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C; LTF; GIG12; LF; anti-LTF antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This LTF antibody is generated from rabbits immunized with LTF recombinant protein.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 666

• Applicable Applications for anti-LTF antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; FC~~1:10~50
• Function: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection. N-terminal region shows strong antifungal activity against C.albicans. Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Isoform DeltaLf: transcription factor with antiproliferative properties and ability to induce cell cycle arrest. Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELENOH promoters.
• Cellular Location: Isoform 1: Secreted. Cytoplasmic granule. Note=Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils.
• Tissue Location: High levels are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine In kidney, detected in the distal collecting tubules in the medulla but not in the cortical region or in blood vessels Detected in peripheral blood neutrophils (at protein level) Isoform 1 and isoform DeltaLf are expressed in breast, prostate, spleen, pancreas, kidney, small intestine, lung, skeletal muscle, uterus, thymus and fetal liver. Isoform 1 is expressed in brain, testis and peripheral blood leukocytes; isoform DeltaLf is barely detectable in these tissues. Isoform DeltaLf is expressed in placenta, liver and ovary; isoform 1 is barely detectable in these tissues. In kidney, isoform 1 is expressed at high levels in the collecting tubules of the medulla but at very low levels in the cortex.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(LTF Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (lefthistogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for theanalysis.)

WB (Western Blot)

(Western blot analysis of LTF Antibody in mouse spleen tissue lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of LTF Antibody in MDA-MB231 cell line lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.)

WB (Western Blot)

(All lanes : Anti-LTF Antibody at 1:1000-2000 dilution Lane 1: Human plasma tissue lysate Lane 2: Hela whole cell lysate Lane 3: Human uterus tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-LTF Antibody at 1:1000 dilution Lane 1: Human plasma tissue lysate Lane 2: Human fetal kidney tissue lysate Lane 3: Human uterus tissue lysate Lane 4: Human skeletal muscle tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

WB (Western Blot)

(All lanes : Anti-LTF Antibody at 1:4000 dilution Lane 1: A549 whole cell lysate Lane 2: Human plasma tissue lysate Lane 3: Human fetal kidney tissue lysate Lane 4: Human breast tissue lysate Lane 5: Human uterus tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-LTF antibody

LTF is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis

Product Categories/Family for anti-LTF antibody

Cancer; Cardiovascular; Signal transduction

References

Willnow,T.E., et.al., J. Biol. Chem. 267 (36), 26172-26180 (1992)

NCBI and Uniprot Product Information

• NCBI GI #: 312433998
• NCBI GeneID: 4057
• NCBI Accession #: NP_001186078.1; NP_002334.2
• NCBI GenBank Nucleotide #: NM_001199149.1
• UniProt Accession #: P02788; O00756; Q16780; Q16785; Q16786; Q16789; Q5DSM0; Q8IU92; A8K9U8; B2MV13; B7Z4X2; E7EQH5
• NCBI Official Full Name: lactotransferrin isoform 2
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12; HEL110
• NCBI Protein Information: lactotransferrin
• UniProt Protein Name: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN