WB (Western Blot)
(Western Blot of human Chymase/CMA1using AAA14718)
Sheep anti-Human Mast Cell Chymase Polyclonal Antibody | anti-CMA1 antibody
Mast Cell Chymase (CMA1)
Gene Names
CMA1; CYH; MCT1; chymase; MGC119890; MGC119891
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Mast Cell Chymase, Antibody; Mast Cell Chymase (CMA1); Anti -Mast Cell Chymase (CMA1); anti-CMA1 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Chymase. Crossreactivity: ~10% with MCPT1 and <1% with rhGranzyme-B, rhGranzyme-H, rmMCPT6 and rmMCPT7.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, ph7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Concentration
~0.2mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-CMA1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation and ELISA.
Dilution: Western Blot: 0.1-0.2ug/ml. Detection limit is ~5ng/lane under non-reducing or reducing conditions.
Immunoprecipitation: Immunoprecipitate rhChymase
from conditioned media of transfected NS0 cells.
ELISA: 0.5-1ug/ml. Detection limit is ~1.5ng/well.
Dilution: Western Blot: 0.1-0.2ug/ml. Detection limit is ~5ng/lane under non-reducing or reducing conditions.
Immunoprecipitation: Immunoprecipitate rhChymase
from conditioned media of transfected NS0 cells.
ELISA: 0.5-1ug/ml. Detection limit is ~1.5ng/well.
Grade
Affinity Purified
Immunogen
Recombinant protein corresponding to Gly20-Asn247 from human Chymase expressed in NS0 cells.
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
WB (Western Blot)
(Western Blot of human Chymase/CMA1using AAA14718)
WB (Western Blot)
(Western Blot of human Chymase/CMA1using AAA14718)
Related Product Information for anti-CMA1 antibody
Chymase (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa22-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa49-247).
Product Categories/Family for anti-CMA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
mast cell chymase
NCBI Official Synonym Full Names
chymase 1, mast cell
NCBI Official Symbol
CMA1
NCBI Official Synonym Symbols
CYH; MCT1; chymase; MGC119890; MGC119891
NCBI Protein Information
chymase; alpha-chymase; chymase, heart; OTTHUMP00000178751; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I
UniProt Protein Name
Chymase
UniProt Gene Name
CMA1
UniProt Synonym Gene Names
CYH; CYM
UniProt Entry Name
CMA1_HUMAN
Similar Products
Product Notes
The CMA1 cma1 (Catalog #AAA14718) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Mast Cell Chymase (CMA1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mast Cell Chymase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot, Immunoprecipitation and ELISA. Dilution: Western Blot: 0.1-0.2ug/ml. Detection limit is ~5ng/lane under non-reducing or reducing conditions. Immunoprecipitation: Immunoprecipitate rhChymase from conditioned media of transfected NS0 cells. ELISA: 0.5-1ug/ml. Detection limit is ~1.5ng/well. Researchers should empirically determine the suitability of the CMA1 cma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mast Cell Chymase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.