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IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens).)
Rabbit MBP Polyclonal Antibody | anti-MBP antibody
MBP Polyclonal Antibody
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
MBP, Antibody; MBP Polyclonal Antibody; MGC99675; MBP; anti-MBP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence Length
304
Applicable Applications for anti-MBP antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IHC: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human Myelin Basic Protein (NP_001020271.1).
Species Reactivity/Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using MBP antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using MBP antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using MBP antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using MBP antibody at dilution of 1:200 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-MBP antibody
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However,MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
Product Categories/Family for anti-MBP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 12-18KDa
Calculated MW: 17kDa/18kDa/20kDa/21kDa/33kDa
Calculated MW: 17kDa/18kDa/20kDa/21kDa/33kDa
NCBI Official Full Name
Myelin basic protein
NCBI Official Synonym Full Names
myelin basic protein
NCBI Official Symbol
MBP
NCBI Protein Information
Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein
UniProt Protein Name
Myelin basic protein
UniProt Gene Name
MBP
UniProt Synonym Gene Names
MBP
UniProt Entry Name
MBP_HUMAN
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Product Notes
The MBP mbp (Catalog #AAA10723) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MBP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the MBP mbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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