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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human, mouse MMP12 Polyclonal Antibody | anti-MMP12 antibody
MMP12 Antibody (C-term)
Gene Names
MMP12; ME; HME; MME; MMP-12
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MMP12, Antibody; MMP12 Antibody (C-term); Macrophage metalloelastase; MME; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12; HME; anti-MMP12 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
391-420
Sequence Length
470
Applicable Applications for anti-MMP12 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-paraffin (IHC-P), Immunofluorescence (IF)
Application Notes
WB~~1:1000
IHC-P~~1:25
IF~~1:10~50
IHC-P~~1:25
IF~~1:10~50
Function
May be involved in tissue injury andremodeling. Has significant elastolyticactivity. Can accept large and small aminoacids at the P1' site, but has a preference forleucine. Aromatic or hydrophobic residues arepreferred at the P1 site, with smallhydrophobic residues (preferably alanine)occupying P3.
Cellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Location
Found in alveolar macrophages but not inperipheral blood monocytes.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse kidney tissue lysates. MMP12 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse kidney tissue lysates. MMP12 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with (lane 2) blocking peptide in 293 cell line lysate. MMP12 (arrow) was detected using the purified Pab;)
WB (Western Blot)
(Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with (lane 2) blocking peptide in 293 cell line lysate. MMP12 (arrow) was detected using the purified Pab;)
WB (Western Blot)
(Anti-MMP12 Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-MMP12 Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-MMP12 Antibody at 1:2000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-MMP12 Antibody at 1:2000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
IHC (Immunohistochemistry)
(Staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Related Product Information for anti-MMP12 antibody
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.
Product Categories/Family for anti-MMP12 antibody
References
Nar, H., et al., J. Mol. Biol. 312(4):743-751 (2001).
Lang, R., et al., J. Mol. Biol. 312(4):731-742 (2001).
Gronski, T.J. Jr., et al., J. Biol. Chem. 272(18):12189-12194 (1997).
Shapiro, S.D., et al., J. Biol. Chem. 268(32):23824-23829 (1993).
Lang, R., et al., J. Mol. Biol. 312(4):731-742 (2001).
Gronski, T.J. Jr., et al., J. Biol. Chem. 272(18):12189-12194 (1997).
Shapiro, S.D., et al., J. Biol. Chem. 268(32):23824-23829 (1993).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
macrophage metalloelastase preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 12
NCBI Official Symbol
MMP12
NCBI Official Synonym Symbols
ME; HME; MME; MMP-12
NCBI Protein Information
macrophage metalloelastase
UniProt Protein Name
Macrophage metalloelastase
UniProt Gene Name
MMP12
UniProt Synonym Gene Names
HME; MME; ME; hME; MMP-12
UniProt Entry Name
MMP12_HUMAN
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Product Notes
The MMP12 mmp12 (Catalog #AAA28713) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 391-420. The MMP12 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MMP12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-paraffin (IHC-P), Immunofluorescence (IF). WB~~1:1000 IHC-P~~1:25 IF~~1:10~50. Researchers should empirically determine the suitability of the MMP12 mmp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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