IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit MMP24 Polyclonal Antibody | anti-MMP24 antibody
MMP24 Antibody (Center)
Gene Names
MMP24; MMP25; MMP-24; MT5MMP; MTMMP5; MT-MMP5; MT5-MMP; MT-MMP 5
Reactivity
HumanPredicted: Mouse, Rat
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MMP24, Antibody; MMP24 Antibody (Center); Matrix metalloproteinase-24; MMP-24; 3424-; Membrane-type matrix metalloproteinase 5; MT-MMP 5; MTMMP5; Membrane-type-5 matrix metalloproteinase; MT5-MMP; MT5MMP; Processed matrix metalloproteinase-24; MMP24; anti-MMP24 antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This MMP24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the Central region of human MMP24.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
396-425
Sequence Length
645
Applicable Applications for anti-MMP24 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
IHC-P~~1:50~100
Function
Metalloprotease that mediates cleavage of N-cadherin (CDH2) and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. Involved in cell-cell interactions between nociceptive neurites and mast cells, possibly by mediating cleavage of CDH2, thereby acting as a mediator of peripheral thermal nociception and inflammatory hyperalgesia. Key regulator of neural stem cells quiescence by mediating cleavage of CDH2, affecting CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate their quiescence. May play a role in axonal growth. Able to activate progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin (By similarity).
Cellular Location
Matrix metalloproteinase-24: Cell membrane; Single-pass type I membrane protein Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Note=Recycled back to the plasma membrane through the trans-Golgi network via interaction with APBA3.
Tissue Location
Predominantly expressed in brain, kidney,pancreas and lung. Overexpressed in a seriesof brain tumors, including astrocytomas and glioblastomas
Calculated MW
73231
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of MMP24 Antibody (Center) in HL60 cell line lysates (35ug/lane). MMP24 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of MMP24 Antibody (Center) in HL60 cell line lysates (35ug/lane). MMP24 (arrow) was detected using the purified Pab.)
Related Product Information for anti-MMP24 antibody
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP24 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP25 but has been renamed matrix metalloproteinase 24 (MMP24).
Product Categories/Family for anti-MMP24 antibody
References
Jung, M., et al., Prostate 55(2):89-98 (2003).
Kajita, M., et al., FEBS Lett. 457(3):353-356 (1999).
Llano, E., et al., Cancer Res. 59(11):2570-2576 (1999).
Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999).
Kinoh, H., et al., Cytogenet. Cell Genet. 87 (1-2), 97-98 (1999).
Kajita, M., et al., FEBS Lett. 457(3):353-356 (1999).
Llano, E., et al., Cancer Res. 59(11):2570-2576 (1999).
Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999).
Kinoh, H., et al., Cytogenet. Cell Genet. 87 (1-2), 97-98 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
matrix metalloproteinase-24 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 24 (membrane-inserted)
NCBI Official Symbol
MMP24
NCBI Official Synonym Symbols
MMP25; MMP-24; MT5MMP; MTMMP5; MT-MMP5; MT5-MMP; MT-MMP 5
NCBI Protein Information
matrix metalloproteinase-24
UniProt Protein Name
Matrix metalloproteinase-24
UniProt Gene Name
MMP24
UniProt Synonym Gene Names
MT5MMP; MMP-24; MT-MMP 5; MTMMP5; MT5-MMP; MT5MMP
UniProt Entry Name
MMP24_HUMAN
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Product Notes
The MMP24 mmp24 (Catalog #AAA28661) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 396-425. The MMP24 Antibody (Center) reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMP24 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB~~1:1000 IHC-P~~1:50~100. Researchers should empirically determine the suitability of the MMP24 mmp24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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