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IHC (Immunohistochemistry)
product-image-AAA11509_WB.jpg

Rabbit anti-Mouse, Rat Myelin Basic Protein Polyclonal Antibody | anti-MBP antibody

Anti-Myelin Basic Protein antibody

Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Synonyms
Myelin Basic Protein, Antibody; Anti-Myelin Basic Protein antibody; Myelin basic protein(MBP); myelin basic protein; GDB antibody; Golli MBP antibody; Hemopoietic MBP antibody; HMBPR antibody; HUGO antibody; MBP antibody; MBP_HUMAN antibody; MGC99675 antibody; MLD antibody; Myelin A1 Protein antibody; Myelin basic protein antibody; Myelin Deficient antibody; Myelin Membrane Encephalitogenic Protein antibody; OTTHUMP00000163776 antibody; OTTHUMP00000174387 antibody; OTTHUMP00000174388 antibody; SHI antibody; Shiverer antibody; SP antibody; anti-MBP antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3
Sequence Length
304
Applicable Applications for anti-MBP antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluoresence (IF)
Application Notes
WB: Concentration 0.1-0.5ug/ml; Tested Species: Ms, Rat
IHC (Paraffin-embedded Section): Concentration: 2-5ug/ml; Tested Species: Ms, Rat; Antigen Retrieval: By Heat
IF: Concentration: 2ug/ml; Tested Species: Ms, Rat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similiarities.
By Heat: Boiling paraffin sections in 10mM citrate buffer pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein (182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-Myelin Basic Protein antibody, AAA11509, IHC(P)IHC(P): Rat Brain Tissue)

WB (Western Blot)

(Anti-Myelin Basic Protein antibody, AAA11509, Western blottingWB: Mouse Brain Tissue Lysate)

Related Product Information for anti-MBP antibody
Description: Rabbit IgG polyclonal antibody for Myelin basic protein(MBP) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Myelin basic protein
NCBI Official Synonym Full Names
myelin basic protein
NCBI Official Symbol
MBP
NCBI Protein Information
Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein
UniProt Protein Name
Myelin basic protein
UniProt Gene Name
MBP
UniProt Synonym Gene Names
MBP
UniProt Entry Name
MBP_HUMAN

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Product Notes

The MBP mbp (Catalog #AAA11509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Myelin Basic Protein antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Myelin Basic Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluoresence (IF). WB: Concentration 0.1-0.5ug/ml; Tested Species: Ms, Rat IHC (Paraffin-embedded Section): Concentration: 2-5ug/ml; Tested Species: Ms, Rat; Antigen Retrieval: By Heat IF: Concentration: 2ug/ml; Tested Species: Ms, Rat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similiarities. By Heat: Boiling paraffin sections in 10mM citrate buffer pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the MBP mbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myelin Basic Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.