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product-image-AAA28824_WB6.jpg WB (Western Blot) (Sample: Cerebrum (Rat) Lysate at 40 ug Raw264.7 (Mouse) Cell Lysate at 30 ug Primary: Anti- NARG2 (bs-5968R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 110 kD)

Rabbit anti-Rat, Dog NARG2 Polyclonal Antibody | anti-NARG2 antibody

NARG2 Polyclonal Antibody

Gene Names
NARG2; BRCC1
Reactivity
Rat, Dog
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NARG2, Antibody; NARG2 Polyclonal Antibody; Little elongation complex subunit 2; Interactor of little elongator complex ELL subunit 2; NMDA receptor-regulated protein 2; ICE2; BRCC1; NARG2; anti-NARG2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-NARG2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Sample: Cerebrum (Rat) Lysate at 40 ug Raw264.7 (Mouse) Cell Lysate at 30 ug Primary: Anti- NARG2 (bs-5968R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 110 kD)

product-image-AAA28824_WB6.jpg WB (Western Blot) (Sample: Cerebrum (Rat) Lysate at 40 ug Raw264.7 (Mouse) Cell Lysate at 30 ug Primary: Anti- NARG2 (bs-5968R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 110 kD)

IHC (Immunohistochemistry)

(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (NARG2) Polyclonal Antibody, Unconjugated (bs-5968R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

product-image-AAA28824_IHC5.jpg IHC (Immunohistochemistry) (Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (NARG2) Polyclonal Antibody, Unconjugated (bs-5968R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

FCM (Flow Cytometry)

(Blank control: HCCLM3(blue) Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5 µl in 100 µL1X PBS containing 0.5% BSA(green).)

product-image-AAA28824_FCM4.jpg FCM (Flow Cytometry) (Blank control: HCCLM3(blue) Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5 µl in 100 µL1X PBS containing 0.5% BSA(green).)

FCM (Flow Cytometry)

(Dilution: Blank control: U-2OS(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µl in 100 µL1X PBS containing 0.5% BSA(green).)

product-image-AAA28824_FCM3.jpg FCM (Flow Cytometry) (Dilution: Blank control: U-2OS(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µl in 100 µL1X PBS containing 0.5% BSA(green).)

FCM (Flow Cytometry)

(Blank control: U-2OS(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µl in 100 µL1X PBS containing 0.5% BSA(green).)

product-image-AAA28824_FCM2.jpg FCM (Flow Cytometry) (Blank control: U-2OS(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µl in 100 µL1X PBS containing 0.5% BSA(green).)

FCM (Flow Cytometry)

(Blank control: RSC96(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µl in 100 µL1X PBS containing 0.5% BSA(green).)

product-image-AAA28824_FCM.jpg FCM (Flow Cytometry) (Blank control: RSC96(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µl in 100 µL1X PBS containing 0.5% BSA(green).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110,011 Da
NCBI Official Full Name
NMDA receptor-regulated protein 2 isoform b
NCBI Official Synonym Full Names
NMDA receptor regulated 2
NCBI Official Symbol
NARG2
NCBI Official Synonym Symbols
BRCC1
NCBI Protein Information
NMDA receptor-regulated protein 2; breast cancer cell 1; NMDA receptor-regulated gene 2
UniProt Protein Name
NMDA receptor-regulated protein 2
UniProt Gene Name
NARG2
UniProt Synonym Gene Names
BRCC1
UniProt Entry Name
NARG2_HUMAN

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Product Notes

The NARG2 narg2 (Catalog #AAA28824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NARG2 Polyclonal Antibody reacts with Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's NARG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF). Researchers should empirically determine the suitability of the NARG2 narg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NARG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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