Goat NOTCH3 Polyclonal Antibody | anti-NOTCH3 antibody

Goat Polyclonal to Human NOTCH3

Cat. Num. AAA12317

• Gene Names: NOTCH3; IMF2; CASIL; CADASIL
• Reactivity: Bat, Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: NOTCH3, Antibody; Goat Polyclonal to Human NOTCH3; Anti-NOTCH3 Antibody (C-Terminus) IHC-plus; NOTCH3; CASIL; CADASIL; Notch 3; Notch (Drosophila) homolog 3; Notch homolog 3; Notch homolog 3 (Drosophila); Human NOTCH3; anti-NOTCH3 antibody

• Host: Goat
• Reactivity: Bat, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human NOTCH3.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 2321

• Applicable Applications for anti-NOTCH3 antibody: Immunohistochemistry (IHC - Paraffin), Peptide Enzyme-Linked Immunosorbent Assay
• Application Notes: IHC - Paraffin (5 µg/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:16000); ; (Optimal dilution to be determined by the researcher)
• Target: NOTCH3
• Immunogen Species: Human
• Immunogen: NOTCH3 antibody was raised against peptide with sequence C-QLGPQPEVTPKRQ, from the C-Terminus of protein sequence according to NP_000426.2
• Family/Subfamily: Fibronectin / Not assigned-Fibronectin
• Epitope: aa2306-2318
• Immunogen Species: NOTCH3 antibody was raised against Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

IHC (Immunohistochemistry)

(Anti-NOTCH3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Product Categories/Family for anti-NOTCH3 antibody

Family: Fibronectin

NCBI and Uniprot Product Information

• NCBI GI #: 134244285
• NCBI GeneID: 4854
• NCBI Accession #: NP_000426.2
• NCBI GenBank Nucleotide #: NM_000435.2
• UniProt Accession #: Q9UM47; Q9UEB3; Q9UPL3; Q9Y6L8
• NCBI Official Full Name: neurogenic locus notch homolog protein 3
• NCBI Official Synonym Full Names: notch 3
• NCBI Official Symbol: NOTCH3
• NCBI Official Synonym Symbols: IMF2; CASIL; CADASIL
• NCBI Protein Information: neurogenic locus notch homolog protein 3; Notch homolog 3
• UniProt Protein Name: Neurogenic locus notch homolog protein 3
• UniProt Gene Name: NOTCH3
• UniProt Synonym Gene Names: Notch 3
• UniProt Entry Name: NOTC3_HUMAN