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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)

Rabbit NPTX2 Polyclonal Antibody | anti-NPTX2 antibody

NPTX2 Antibody

Gene Names
NPTX2; NP2; NARP; NP-II
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
NPTX2 Antibody is affinity chromatography purified via peptide column.
Synonyms
NPTX2, Antibody; NPTX2 Antibody; NPTX2; NP2; NARP; NP-II; Neuronal pentraxin-2; Neuronal pentraxin II; neuronal pentraxin II; anti-NPTX2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
NPTX2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
431
Applicable Applications for anti-NPTX2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)
Application Notes
WB: 0.5-4 ug/mL
IHC: 5 ug/mL
IF: 20 ug/mL

Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human and mouse samples; Immunofluorescence in humanand mouse samples. All other applications and species not yet tested.
Conjugate
Unconjugated
Immunogen
NPTX2 antibody was raised against a 16 amino acid synthetic peptide near the center of the human NPTX2.
The immunogen is located within amino acids 170-220 of NPTX2.
Homology
Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
Validation
Independent Antibody Validation in Cell lines shows similar NPTX2expression profile in human and mouse cell lines detected by two independent anti-NPTX2 antibodies that recognize different epitopes, AAA10933 against central domain. NPTX2 proteins are detected only in HeLa and HepG2 cell lines by the two independent antibodies.
Isoforms
Human NPTX2 has one isoform (431aa, 47kD). Mouse NPTX2 has one isoform (429aa,47.1kD) and Rat NPTX2 also has one isoform (432aa, 47.4kD). 4573 can detect human,mouse and rat
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)

IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)

WB (Western Blot)

(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.)

WB (Western Blot) (Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.)

Application Data

(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933(2 ug/mL) and beta-actin (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Application Data (Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933(2 ug/mL) and beta-actin (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

IF (Immunofluorescence)

(Immunofluorescence of NPTX2 in mouse brain tissue with NPTX2 antibody at 20 μg/mL.Red: NPTX2 Antibody (4573)Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of NPTX2 in mouse brain tissue with NPTX2 antibody at 20 μg/mL.Red: NPTX2 Antibody (4573)Blue: DAPI staining)

WB (Western Blot)

(Western blot analysis of NPTX2 expression in mouse brain tissue lysate with NPTX2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

WB (Western Blot) (Western blot analysis of NPTX2 expression in mouse brain tissue lysate with NPTX2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (AAA10933) at 5ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (AAA10933) at 5ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IF (Immunofluorescence)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)

IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (AAA10933) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (AAA10933) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot)

(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933 (A: 0.5 ug/mL, B: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at)

WB (Western Blot) (Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933 (A: 0.5 ug/mL, B: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at)
Related Product Information for anti-NPTX2 antibody
NPTX2 Antibody: Neuronal pentraxin (NPTX) 2 was initially identified as a member of the pentraxin family having high homology to NPTX1. Unlike NPTX1 however, NPTX2 is expressed in testes, pancreas, liver, heart, skeletal muscle as well as brain. NPTX2 possesses the lectin properties common to the pentraxin family, promotes neurite outgrowth, and is rapidly regulated by neuronal activity. NPTX1 and NPTX2 form heterocomplexes that contribute to both activity-independent and -dependent excitatory synaptogenesis. Recently, NPTX2 has been found to be highly upregulated in Parkinsonian substantia nigra and localizes to Lewy bodies and Lewy neurites in sporadic Parkinson's disease (PD), suggesting that it is involved in the pathway dysregulation that underlies PD. This NPTX2 antibody is predicted to be specific to NPTX2 and not recognize NPTX1.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted MW: 47 KD
Observed MW: 47 KD
NCBI Official Full Name
neuronal pentraxin-2
NCBI Official Synonym Full Names
neuronal pentraxin II
NCBI Official Symbol
NPTX2
NCBI Official Synonym Symbols
NP2; NARP; NP-II
NCBI Protein Information
neuronal pentraxin-2; apexin; neuronal activity-regulated pentaxin
UniProt Protein Name
Neuronal pentraxin-2
UniProt Gene Name
NPTX2
UniProt Synonym Gene Names
NP2; NP-II
UniProt Entry Name
NPTX2_HUMAN

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Product Notes

The NPTX2 nptx2 (Catalog #AAA10933) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPTX2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NPTX2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF). WB: 0.5-4 ug/mL IHC: 5 ug/mL IF: 20 ug/mL Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human and mouse samples; Immunofluorescence in humanand mouse samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the NPTX2 nptx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPTX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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