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Rabbit PERK Polyclonal Antibody | anti-PERK antibody
PERK Polyclonal Antibody
Gene Names
EIF2AK3; PEK; WRS; PERK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Synonyms
PERK, Antibody; PERK Polyclonal Antibody; EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; anti-PERK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Applicable Applications for anti-PERK antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Application Notes
IF: 1:50-200
WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/40000
WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/40000
Cellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location
Ubiquitous. A high level expression is seen in secretory tissues
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
IF (Immunofluorescence)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
IF (Immunofluorescence)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
IF (Immunofluorescence)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
Application Data
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
Application Data
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
IF (Immunofluorescence)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
Related Product Information for anti-PERK antibody
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)- induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125,216 Da
NCBI Official Full Name
eukaryotic translation initiation factor 2-alpha kinase 3
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2-alpha kinase 3
NCBI Official Symbol
EIF2AK3
NCBI Official Synonym Symbols
PEK; WRS; PERK
NCBI Protein Information
eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; pancreatic EIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; eukaryotic translation initiation factor 2 alpha kinase 3
UniProt Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 3
UniProt Gene Name
EIF2AK3
UniProt Synonym Gene Names
PEK; PERK; HsPEK
UniProt Entry Name
E2AK3_HUMAN
