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IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PEX10 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Rabbit PEX10 Polyclonal Antibody | anti-PEX10 antibody
PEX10 (CT) Antibody
Gene Names
PEX10; NALD; PBD6A; PBD6B; RNF69
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
PEX10 Antibody is affinity chromatography purified via peptide column.
Synonyms
PEX10, Antibody; PEX10 (CT) Antibody; PEX10 Antibody: Peroxin-10; Peroxisomal biogenesis factor 10; Peroxisome assembly protein 10; RING finger protein 69; RNF69; anti-PEX10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
PEX10 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-PEX10 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 4ug/mL; IF: 20ug/mL
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Immunogen
Anti-PEX10 antibody (9671) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10.
The immunogen is located within last 50 amino acids of PEX10.
The immunogen is located within last 50 amino acids of PEX10.
Homology
Predicted species reactivity based on immunogen sequence: monkey (100%), horse (100%), gorilla (100%), chimpanzee (100%)
Research Area
Signal Transduction
NCBI Organism
Homo sapiens
Isoforms
Human PEX10 has 2 isoforms, including isoform 1 (326aa, 37.1kD) and isoform 2 (346aa, 39.2kD). Mouse PEX10 has 1 isoform (324aa, 37.2kD). can detect PEX10 in human and mouse.
Preparation and Storage
PEX10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PEX10 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PEX10 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of PEX10 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of PEX10 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 3 WB Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: PEX10 AAA11049, 4ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 3 WB Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: PEX10 AAA11049, 4ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 WB Validation in Mouse Tissues Loading: 15ug of lysates per lane.Antibodies: PEX10 AAA11049, 4ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 WB Validation in Mouse Tissues Loading: 15ug of lysates per lane.Antibodies: PEX10 AAA11049, 4ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 WB Validation in Human and Mouse Cell LinesLoading: 15ug of lysate Antibodies: PEX10 AAA11049, 4ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 WB Validation in Human and Mouse Cell LinesLoading: 15ug of lysate Antibodies: PEX10 AAA11049, 4ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)
Related Product Information for anti-PEX10 antibody
PEX10 is implicated in the biogenesis of peroxisomes. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome.
Product Categories/Family for anti-PEX10 antibody
References
Warren D.S. et al. Am. J. Hum. Genet. 63:347-359(1998)
Okumoto K. et al. Hum. Mol. Genet. 7:1399-1405(1998)
Ota T. et al. Nat. Genet. 36:40-45(2004)
Okumoto K. et al. Hum. Mol. Genet. 7:1399-1405(1998)
Ota T. et al. Nat. Genet. 36:40-45(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,069 Da
NCBI Official Full Name
peroxisome biogenesis factor 10 isoform 2
NCBI Official Synonym Full Names
peroxisomal biogenesis factor 10
NCBI Official Symbol
PEX10
NCBI Official Synonym Symbols
NALD; PBD6A; PBD6B; RNF69
NCBI Protein Information
peroxisome biogenesis factor 10; peroxin 10; peroxin-10; RING finger protein 69; peroxisome assembly protein 10
UniProt Protein Name
Peroxisome biogenesis factor 10
UniProt Gene Name
PEX10
UniProt Synonym Gene Names
RNF69
UniProt Entry Name
PEX10_HUMAN
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Product Notes
The PEX10 pex10 (Catalog #AAA11049) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEX10 (CT) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PEX10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 4ug/mL; IF: 20ug/mL Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the PEX10 pex10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEX10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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