DB (Dot Blot)
(Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit Phospho-AKT3 (S472) Polyclonal Antibody | anti-AKT3 antibody
Phospho-AKT3 (S472) Antibody
Gene Names
AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Reactivity
Human Predicted: Mouse, Rat.
Applications
Dot Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-AKT3 (S472), Antibody; Phospho-AKT3 (S472) Antibody; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; anti-AKT3 antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse, Rat.
Predicted: Mouse, Rat.
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AKT3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S472 of human AKT3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
465
Applicable Applications for anti-AKT3 antibody
Dot Blot (DB), ELISA (EIA)
Application Notes
DB~~1:500
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles .
DB (Dot Blot)
(Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Related Product Information for anti-AKT3 antibody
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
Function: AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis.!!Cellular location||Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation.!!Tissue location||In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not atall in kidney.
Function: AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis.!!Cellular location||Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation.!!Tissue location||In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not atall in kidney.
Product Categories/Family for anti-AKT3 antibody
References
Xu, Z., et al., Biochem. Biophys. Res. Commun. 312(2):388-396 (2003).
Tiwari, G., et al., Mol. Cancer Res. 1(6):475-484 (2003).
Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003).
Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002).
Brodbeck, D., et al., J. Biol. Chem. 276(31):29550-29558 (2001).
Tiwari, G., et al., Mol. Cancer Res. 1(6):475-484 (2003).
Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003).
Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002).
Brodbeck, D., et al., J. Biol. Chem. 276(31):29550-29558 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
RAC-gamma serine/threonine-protein kinase isoform 2
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 3
NCBI Official Symbol
AKT3
NCBI Official Synonym Symbols
MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
NCBI Protein Information
RAC-gamma serine/threonine-protein kinase
UniProt Protein Name
RAC-gamma serine/threonine-protein kinase
UniProt Gene Name
AKT3
UniProt Synonym Gene Names
PKBG; PKB gamma
UniProt Entry Name
AKT3_HUMAN
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Product Notes
The AKT3 akt3 (Catalog #AAA28777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-AKT3 (S472) Antibody reacts with Human Predicted: Mouse, Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-AKT3 (S472) can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA). DB~~1:500. Researchers should empirically determine the suitability of the AKT3 akt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-AKT3 (S472), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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