Rabbit PI-16/PI16 Polyclonal Antibody | anti-PI16 antibody

Anti-PI-16/PI16 Antibody

Cat. Num. AAA19330

• Gene Names: PI16; PSPBP; CRISP9; MSMBBP
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: PI-16/PI16, Antibody; Anti-PI-16/PI16 Antibody; PI16; CRISP9; PSPBP; PSEC0164; UNQ289/PRO328; Peptidase inhibitor 16; PI-16; Cysteine-rich secretory protein 9; CRISP-9; PSP94-binding protein; CD antigen CD364; peptidase inhibitor 16; anti-PI16 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for PI-16/PI16 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

• Applicable Applications for anti-PI16 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Human, Mouse, Rat, Monkey|; IHC-P: 2-5ug/ml|Human, Mouse, Rat|; FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human PI-16/PI16 recombinant protein (Position: L28-L363).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 8. Flow Cytometry analysis of HEPA1-6 cells using anti-PI-16/PI16 antibody (AAA19330).Overlay histogram showing HEPA1-6 cells stained with AAA19330 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI-16/PI16 Antibody (AAA19330, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry)

(Figure 7. Flow Cytometry analysis of A431 cells using anti-PI-16/PI16 antibody (AAA19330).Overlay histogram showing A431 cells stained with AAA19330 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI-16/PI16 Antibody (AAA19330, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry)

(Figure 6. IHC analysis of PI-16/PI16 using anti-PI-16/PI16 antibody (AAA19330).PI-16/PI16 was detected in paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PI-16/PI16 Antibody (AAA19330) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of PI-16/PI16 using anti-PI-16/PI16 antibody (AAA19330).PI-16/PI16 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PI-16/PI16 Antibody (AAA19330) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of PI-16/PI16 using anti-PI-16/PI16 antibody (AAA19330).PI-16/PI16 was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PI-16/PI16 Antibody (AAA19330) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of PI-16/PI16 using anti-PI-16/PI16 antibody (AAA19330).PI-16/PI16 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PI-16/PI16 Antibody (AAA19330) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of PI-16/PI16 using anti-PI-16/PI16 antibody (AAA19330).PI-16/PI16 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PI-16/PI16 Antibody (AAA19330) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of PI-16/PI16 using anti-PI-16/PI16 antibody (AAA19330).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: monkey heart tissue lysatesLane 5: rat heart tissue lysatesLane 6: rat testis tissue lysatesLane 7: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PI-16/PI16 antigen affinity purified polyclonal antibody (Catalog # AAA19330) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for PI-16/PI16 at approximately 70-75KD. The expected band size for PI-16/PI16 is at 70-75KD.)

Related Product Information for anti-PI16 antibody

Peptidase inhibitor 16 is a protein that in humans is encoded by the PI16 gene. PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator.

References

1. Eeles, R. A., Kote-Jarai, Z., Giles, G. G., Al Olama, A. A., Guy, M., Jugurnauth, S. K., Mulholland, S., Leongamornlert, D. A., Edwards, S. M., Morrison, J., Field, H. I., Southey, M. C., and 37 others. Multiple newly identified loci associated with prostate cancer susceptibility. Nature Genet. 40: 316-321, 2008.
2. Buckland, P. R., Hoogendoorn, B., Coleman, S. L., Guy, C. A., Smith, S. K., O'Donovan, M. C. Strong bias in the location of functional promoter polymorphisms. Hum. Mutat. 26: 214-223, 2005.
3. Lou, H., Yeager, M., Li, H., Bosquet, J. G., Hayes, R. B., Orr, N., Yu, K., Hutchinson, A., Jacobs, K. B., Kraft, P., Wacholder, S., Chatterjee, N., and 24 others. Fine mapping and functional analysis of a common variant in MSMB on chromosome 10q11. 2 associated with prostate cancer susceptibility. Proc. Nat. Acad. Sci. 106: 7933-7938, 2009.

NCBI and Uniprot Product Information

• NCBI GI #: 312434019
• NCBI GeneID: 221476
• NCBI Accession #: NP_001186088.1
• NCBI GenBank Nucleotide #: NM_001199159.1
• UniProt Accession #: Q6UXB8; Q6ZVG9; Q8IYL8; Q8NBK0; Q8TCB8
• Molecular Weight: 29,674 Da
• NCBI Official Full Name: peptidase inhibitor 16
• NCBI Official Synonym Full Names: peptidase inhibitor 16
• NCBI Official Symbol: PI16
• NCBI Official Synonym Symbols: PSPBP; CRISP9; MSMBBP
• NCBI Protein Information: peptidase inhibitor 16; PSP94-binding protein; cysteine-rich secretory protein 9; microseminoprotein, beta-binding protein; protease inhibitor 16
• UniProt Protein Name: Peptidase inhibitor 16
• UniProt Gene Name: PI16
• UniProt Synonym Gene Names: CRISP9; PSPBP; PI-16; CRISP-9
• UniProt Entry Name: PI16_HUMAN