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WB (Western Blot)
(Anti-PIAS1 antibody, AAA11605, All Western blottingAll lanes: Anti-PIAS1(AAA11605) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Mouse Testis Tissue Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD)
Rabbit PIAS1 Polyclonal Antibody | anti-PIAS1 antibody
Anti-PIAS1 antibody
Gene Names
PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
PIAS1, Antibody; Anti-PIAS1 antibody; E3 SUMO-protein ligase PIAS1; protein inhibitor of activated STAT, 1; DDXBP1 antibody; DEAD/H box binding protein 1 antibody; DEAD/H box-binding protein 1 antibody; E3 SUMO-protein ligase PIAS1 antibody; GBP antibody; Gu binding protein antibody; Gu-binding protein antibody; Pias1 antibody; PIAS1_HUMAN antibody; Protein inhibitor of activated STAT protein 1 antibody; RNA helicase II binding protein antibody; RNA helicase II-binding protein antibody; anti-PIAS1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
651
Applicable Applications for anti-PIAS1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1 (636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-PIAS1 antibody, AAA11605, All Western blottingAll lanes: Anti-PIAS1(AAA11605) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Mouse Testis Tissue Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD)
WB (Western Blot)
(Anti-PIAS1 antibody, AAA11605, All Western blottingAll lanes: Anti-PIAS1(AAA11605) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Mouse Testis Tissue Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Rat Testis Tissue)
IHC (Immunohistochemistry)
(Anti-PIAS1 antibody, AAA11605, IHC(P)IHC(P): Rat Testis Tissue)
ICC (Immunocytochemistry)
(Anti-PIAS1 antibody, AAA11605, ICCICC: A549 Cell)
ICC (Immunocytochemistry)
(Anti-PIAS1 antibody, AAA11605, ICCICC: A549 Cell)
Related Product Information for anti-PIAS1 antibody
Description: Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.
Background: E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
72,203 Da
NCBI Official Full Name
E3 SUMO-protein ligase PIAS1
NCBI Official Synonym Full Names
protein inhibitor of activated STAT, 1
NCBI Official Symbol
PIAS1
NCBI Official Synonym Symbols
GBP; ZMIZ3; DDXBP1; GU/RH-II
NCBI Protein Information
E3 SUMO-protein ligase PIAS1; gu-binding protein; AR interacting protein; DEAD/H box-binding protein 1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3; protein inhibitor of activated STAT-1; protein inhibitor of activated STAT protein 1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
UniProt Protein Name
E3 SUMO-protein ligase PIAS1
UniProt Gene Name
PIAS1
UniProt Synonym Gene Names
DDXBP1; GBP
UniProt Entry Name
PIAS1_HUMAN
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Product Notes
The PIAS1 pias1 (Catalog #AAA11605) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PIAS1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIAS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the PIAS1 pias1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIAS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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