Goat PNPLA3/Adiponutrin Polyclonal Antibody | anti-PNPLA3 antibody

Goat anti-PNPLA3/Adiponutrin Antibody

Cat. Num. AAA13661

• Gene Names: Pnpla3; Adpn
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PNPLA3/Adiponutrin, Antibody; Goat anti-PNPLA3/Adiponutrin Antibody; Pnpla3; patatin-like phospholipase domain containing 3; Adpn; adiponutrin; Pnpla3 antibody; patatin-like phospholipase domain containing 3 antibody; Adpn antibody; adiponutrin antibody; Adiponutrin; PNPLA3 / Adiponutrin; anti-PNPLA3 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EHDICPKVKSTN
• Sequence Length: 384

• Applicable Applications for anti-PNPLA3 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Immunohistochemistry: In paraffin embedded Human Skin shows membranous staining of basak kayer cells.; Recommended concentration, 4-6ug/ml.; Western Blot: Approx 45kDa band observed in Mouse Adrenal gland lysates; (calculated MW of 42.6kDa according to Mouse NP_473429.2); Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room tempeture.; IHC: Paraffin embedded Human Kidney. Recommended concentration: 8ug/mL.
• Immunogen: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.
• Note: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IHC (Immunohistochemistry)

(Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.)

IHC (Immunohistochemistry)

((8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

IHC (Immunohistochemistry)

((3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot)

((0. 01ug/ml) staining of Mouse (A) and Rat (B) Adrenal gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot)

((0. 05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 144226245
• NCBI GeneID: 116939
• NCBI Accession #: NP_473429.2
• NCBI GenBank Nucleotide #: NM_054088.3
• NCBI Official Full Name: patatin-like phospholipase domain-containing protein 3
• NCBI Official Synonym Full Names: patatin-like phospholipase domain containing 3
• NCBI Official Symbol: Pnpla3
• NCBI Official Synonym Symbols: Adpn
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 3
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 3
• UniProt Gene Name: Pnpla3
• UniProt Synonym Gene Names: Adpn; iPLA2-epsilon
• UniProt Entry Name: PLPL3_MOUSE