KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: PSMD9 knockout A549 cell lysate; Predicted MW: 24,22,13kd Observed MW: 30kd Primary Ab: 5ug/ml Rabbit Anti-Human PSMD9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit anti-Human, Mouse Proteasome 26S Subunit Polyclonal Antibody | anti-PSMD9 antibody
Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 9 (PSMD9)
Gene Names
PSMD9; p27; Rpn4
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Proteasome 26S Subunit, Antibody; Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 9 (PSMD9); anti-PSMD9 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PSMD9. It has beenselected for its ability to recognize PSMD9 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SDEEARQSG GSSQAGVVTV SDVQELMRRK EEIEAQIKAN YDVLESQKGI GMNEPLVDCE GYPRSDVDLY QVRTARHNII CLQNDHKAVM KQVEEALHQL HARDKEKQAR DMAEAHKEAM SRKLGQSESQ GPPRAFAKVN SISPGSPASI AGLQVDDEIV EFGSVNTQNF QSLHNIGSVV QHSEGKPLNV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SDEEARQSG GSSQAGVVTV SDVQELMRRK EEIEAQIKAN YDVLESQKGI GMNEPLVDCE GYPRSDVDLY QVRTARHNII CLQNDHKAVM KQVEEALHQL HARDKEKQAR DMAEAHKEAM SRKLGQSESQ GPPRAFAKVN SISPGSPASI AGLQVDDEIV EFGSVNTQNF QSLHNIGSVV QHSEGKPLNV
Applicable Applications for anti-PSMD9 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-5ug/mL
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
PSMD9 (Ser2~Arg223)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant PSMD9 (Ser2~Arg223) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: PSMD9 knockout A549 cell lysate; Predicted MW: 24,22,13kd Observed MW: 30kd Primary Ab: 5ug/ml Rabbit Anti-Human PSMD9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: PSMD9 knockout A549 cell lysate; Predicted MW: 24,22,13kd Observed MW: 30kd Primary Ab: 5ug/ml Rabbit Anti-Human PSMD9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Mouse Spleen Tissue; Lane3: Mouse Heart Tissue; Lane4: Human A549 Cells; Lane5: Mouse Placenta Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Mouse Spleen Tissue; Lane3: Mouse Heart Tissue; Lane4: Human A549 Cells; Lane5: Mouse Placenta Tissue.)
Product Categories/Family for anti-PSMD9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,053 Da
NCBI Official Full Name
26S proteasome non-ATPase regulatory subunit 9 isoform 2
NCBI Official Synonym Full Names
proteasome 26S subunit, non-ATPase 9
NCBI Official Symbol
PSMD9
NCBI Official Synonym Symbols
p27; Rpn4
NCBI Protein Information
26S proteasome non-ATPase regulatory subunit 9
UniProt Protein Name
26S proteasome non-ATPase regulatory subunit 9
UniProt Gene Name
PSMD9
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Product Notes
The PSMD9 psmd9 (Catalog #AAA20884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 9 (PSMD9) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Proteasome 26S Subunit can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunocytochemistry in formalin fixed cells: 5-20ug/mL Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL Immunohistochemistry in paraffin section: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PSMD9 psmd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SDEEARQSG GSSQAGVVTV SDVQELMRRK EEIEAQIKAN YDVLESQKGI GMNEPLVDCE GYPRSDVDLY QVRTARHNII CLQNDHKAVM KQVEEALHQL HARDKEKQAR DMAEAHKEAM SRKLGQSESQ GPPRAFAKVN SISPGSPASI AGLQVDDEIV EFGSVNTQNF QSLHNIGSVV QHSEGKPLNV. It is sometimes possible for the material contained within the vial of "Proteasome 26S Subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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