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IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Rev-Erbα/NR1D1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit Rev-Erbalpha/NR1D1 Polyclonal Antibody | anti-NR1D1 antibody
Rev-Erbalpha/NR1D1 Rabbit pAb
Gene Names
NR1D1; EAR1; hRev; THRA1; THRAL; ear-1
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity purification
Synonyms
Rev-Erbalpha/NR1D1, Antibody; Rev-Erbalpha/NR1D1 Rabbit pAb; NR1D1; EAR1; THRA1; THRAL; ear-1; hRev; nuclear receptor subfamily 1 group D member 1; anti-NR1D1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-NR1D1 antibody
Western Blot (WB), Immunohistochemistry (IHC-p), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 280-360 of human Rev-Erb?/NR1D1 (NP_068370.1).
Species Reactivity/Cross Reactivity
Human,Mouse,Rat
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Rev-Erbα/NR1D1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Rev-Erbα/NR1D1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Rev-Erbα/NR1D1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Rev-Erbα/NR1D1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat liver using Rev-Erbα/NR1D1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat liver using Rev-Erbα/NR1D1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using Rev-Erbα/NR1D1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using Rev-Erbα/NR1D1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of lysates from Rat lung, using Rev-Erbα/NR1D1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of lysates from Rat lung, using Rev-Erbα/NR1D1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of lysates from HepG2 cells, using Rev-Erbα/NR1D1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s)
WB (Western Blot)
(Western blot analysis of lysates from HepG2 cells, using Rev-Erbα/NR1D1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s)
Related Product Information for anti-NR1D1 antibody
Background: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes.
Product Categories/Family for anti-NR1D1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 78kDa
alculated MW: 67kDa
alculated MW: 67kDa
NCBI Official Full Name
Nuclear receptor subfamily 1 group D member 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 1, group D, member 1
NCBI Official Symbol
NR1D1
NCBI Official Synonym Symbols
EAR1; hRev; THRA1; THRAL; ear-1
NCBI Protein Information
nuclear receptor subfamily 1 group D member 1; Rev-ErbAalpha; rev-erbA-alpha; V-erbA-related protein 1; nuclear receptor Rev-ErbA-alpha
UniProt Protein Name
Nuclear receptor subfamily 1 group D member 1
UniProt Gene Name
NR1D1
UniProt Synonym Gene Names
EAR1; HREV; THRAL; EAR-1
UniProt Entry Name
NR1D1_HUMAN
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Product Notes
The NR1D1 nr1d1 (Catalog #AAA28372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rev-Erbalpha/NR1D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-p), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200. Researchers should empirically determine the suitability of the NR1D1 nr1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rev-Erbalpha/NR1D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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