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IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells cells using AAA26933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human, mouse S1PR1 Polyclonal Antibody | anti-S1PR1 antibody
S1PR1 Antibody
Gene Names
S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
Reactivity
Human, mouse
Applications
ELISA, Immunohistochemistry, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
S1PR1, Antibody; S1PR1 Antibody; Sphingosine 1-phosphate receptor 1 (S1P receptor 1) (S1P1) (Endothelial differentiation G-protein coupled receptor 1) (Sphingosine 1-phosphate receptor Edg-1) (S1P receptor Edg-1) (CD antigen CD363), S1PR1, CHEDG1 EDG1 ; anti-S1PR1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Applicable Applications for anti-S1PR1 antibody
ELISA (EIA), Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Sphingosine 1-phosphate receptor 1 protein (311-382AA)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-31625 / sc-16070 / sc-25489
Species
Homo sapiens (Human)
Research Area
Cell Biology
Target Names
S1PR1
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells cells using AAA26933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells cells using AAA26933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA26933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA26933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA26933 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA26933 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA26933 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA26933 at dilution 1:100)
WB (Western Blot)
(Western blotAll lanes: S1PR1 antibody at 2ug/mlLane 1: Jurkat whole cell lysateLane 2: A375 whole cell lysateLane 3: Mouse thymus tissueLane 4: HepG2 cellsSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 43kDaObserved band size: 43kDa)
WB (Western Blot)
(Western blotAll lanes: S1PR1 antibody at 2ug/mlLane 1: Jurkat whole cell lysateLane 2: A375 whole cell lysateLane 3: Mouse thymus tissueLane 4: HepG2 cellsSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 43kDaObserved band size: 43kDa)
Related Product Information for anti-S1PR1 antibody
G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
sphingosine 1-phosphate receptor 1
NCBI Official Synonym Full Names
sphingosine-1-phosphate receptor 1
NCBI Official Symbol
S1PR1
NCBI Official Synonym Symbols
EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
NCBI Protein Information
sphingosine 1-phosphate receptor 1
UniProt Protein Name
Sphingosine 1-phosphate receptor 1
UniProt Gene Name
S1PR1
UniProt Synonym Gene Names
CHEDG1; EDG1; S1P receptor 1; S1P1; S1P receptor Edg-1
UniProt Entry Name
S1PR1_HUMAN
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Product Notes
The S1PR1 s1pr1 (Catalog #AAA26933) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S1PR1 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's S1PR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the S1PR1 s1pr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S1PR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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