FCM (Flow Cytometry)
(SERPINH1 Antibody (Center) (Cat. #AAA28730) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit SERPINH1 Polyclonal Antibody | anti-SERPINH1 antibody
SERPINH1 Antibody (Center)
Gene Names
SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SERPINH1, Antibody; SERPINH1 Antibody (Center); Serpin H1; 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47; SERPINH1; CBP1; CBP2; HSP47; SERPINH2; anti-SERPINH1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This SERPINH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-196 amino acids from the Central region of human SERPINH1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
169-196
Sequence Length
418
Applicable Applications for anti-SERPINH1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-P (IHC-P), Flow Cytometry (FC)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Function
Binds specificially to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Cellular Location
Endoplasmic reticulum lumen.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM (Flow Cytometry)
(SERPINH1 Antibody (Center) (Cat. #AAA28730) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(SERPINH1 Antibody (Center) (Cat. #AAA28730) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(SERPINH1 Antibody (Center) (RB18952) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SERPINH1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(SERPINH1 Antibody (Center) (RB18952) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SERPINH1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of SERPINH1 antibody (Center) (Cat#AAA28730) in HepG2 cell line lysates (35ug/lane). SERPINH1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SERPINH1 antibody (Center) (Cat#AAA28730) in HepG2 cell line lysates (35ug/lane). SERPINH1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-SERPINH1 antibody
SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.
Product Categories/Family for anti-SERPINH1 antibody
References
Araki,K., Br. J. Cancer 101 (3), 492-497 (2009)
Katagata,Y., J. Dermatol. Sci. 49 (1), 33-38 (2008)
Katagata,Y., J. Dermatol. Sci. 49 (1), 33-38 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46441
NCBI Official Full Name
serpin H1
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
NCBI Official Symbol
SERPINH1
NCBI Official Synonym Symbols
CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
NCBI Protein Information
serpin H1
UniProt Protein Name
Serpin H1
UniProt Gene Name
SERPINH1
UniProt Synonym Gene Names
CBP1; CBP2; HSP47; SERPINH2; AsTP3; Colligin
UniProt Entry Name
SERPH_HUMAN
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Product Notes
The SERPINH1 serpinh1 (Catalog #AAA28730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 169-196. The SERPINH1 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-P (IHC-P), Flow Cytometry (FC). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the SERPINH1 serpinh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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