Rabbit anti-Human SGPP1 Polyclonal Antibody | anti-Sgpp1 antibody

SGPP1, NT (SGPP1, Sphingosine-1-phosphate phosphatase 1, Sphingosine-1-phosphatase 1)

Cat. Num. AAA24046

• Gene Names: Sgpp1; SPP; SPP1; Spph1; AI463453
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: SGPP1, Antibody; SGPP1, NT (SGPP1, Sphingosine-1-phosphate phosphatase 1, Sphingosine-1-phosphatase 1); Anti -SGPP1, NT (SGPP1, Sphingosine-1-phosphate phosphatase 1, Sphingosine-1-phosphatase 1); anti-Sgpp1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SGPP1
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
• Concentration: 0.47 mg/mL (varies by lot)

• Applicable Applications for anti-Sgpp1 antibody: ELISA (EIA), Western Blot (WB); Immunohistochemistry (Formalin fixed paraffin-embedded); Immunohistochemistry (Formalin fixed paraffin embedded, Leica); Other applications not yet tested.
• Application Notes: WB: 1:1000; IHC (FFPE): 1:10-50; IHC (FFPE, Leica):1:500 Requires HIER using EDTA buffer , pH9.0 prior to staining; Optimal dilutions/concentrations should be determined by the end user.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 13-42 in the N-terminal region of human SGPP1.
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

WB (Western Blot)

(Western Blot analysis using AAA24046 (1:1000)Lane 1: HACAT whole cell lysate Lane 2: A431 whole cell lysateLysate prteins: 20 ug/lane.Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10000). Predicted bamd size: 49 kDBlocking/Dilution buffer: 5% NFDM/TBST)

WB (Western Blot)

(Western Blot analysis of human placenta lysate (:20ug/lane) using AAA24046 (1:1000)Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10000). Predicted bamd size: 49 kDBlocking/Dilution buffer: 5% NFDM/TBST)

IHC (Immunohistochemistry)

(SGPP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SGPP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(SGPP1 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SGPP1 antibody detected the SGPP1 protein (arrow).)

Related Product Information for anti-Sgpp1 antibody

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM].

Product Categories/Family for anti-Sgpp1 antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 13507712
• NCBI GeneID: 81535
• NCBI Accession #: NP_110418.1
• NCBI GenBank Nucleotide #: NM_030750.3
• UniProt Accession #: Q9BX95; Q9JI99
• NCBI Official Full Name: sphingosine-1-phosphate phosphatase 1
• NCBI Official Synonym Full Names: sphingosine-1-phosphate phosphatase 1
• NCBI Official Symbol: Sgpp1
• NCBI Official Synonym Symbols: SPP; SPP1; Spph1; AI463453
• NCBI Protein Information: sphingosine-1-phosphate phosphatase 1; SPPase1; sphingosine-1-phosphatase 1
• UniProt Protein Name: Sphingosine-1-phosphate phosphatase 1
• UniProt Gene Name: Sgpp1
• UniProt Synonym Gene Names: Spp1; Spph1; SPP; SPPase1; mSPP1
• UniProt Entry Name: SGPP1_MOUSE