IHC (Immunohistochemistry)
(Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit SHARP1 Polyclonal Antibody | anti-BHLHE41 antibody
Anti-SHARP1 Antibody
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
SHARP1, Antibody; Anti-SHARP1 Antibody; BHLHB3; DEC2; SHARP1; Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1; anti-BHLHE41 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of SHARP1 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
482
Applicable Applications for anti-BHLHE41 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000)
IHC (1/100 - 1/200)
IHC (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SHARP1. The exact sequence is proprietary.
Preparation and Storage
Upon receipt, aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of SHARP1 expression in SHSY5Y (A), RAW264.7 (B), PC12 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 50 kD).)
WB (Western Blot)
(Western blot analysis of SHARP1 expression in SHSY5Y (A), RAW264.7 (B), PC12 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 50 kD).)
Related Product Information for anti-BHLHE41 antibody
Rabbit polyclonal antibody to SHARP1
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
class E basic helix-loop-helix protein 41
NCBI Official Synonym Full Names
basic helix-loop-helix family, member e41
NCBI Official Symbol
BHLHE41
NCBI Official Synonym Symbols
DEC2; hDEC2; BHLHB3; SHARP1
NCBI Protein Information
class E basic helix-loop-helix protein 41
UniProt Protein Name
Class E basic helix-loop-helix protein 41
UniProt Gene Name
BHLHE41
UniProt Synonym Gene Names
BHLHB3; DEC2; SHARP1; bHLHe41; bHLHb3; hDEC2; SHARP-1
UniProt Entry Name
BHE41_HUMAN
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Product Notes
The BHLHE41 bhlhe41 (Catalog #AAA27795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SHARP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHARP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000) IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the BHLHE41 bhlhe41 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHARP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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