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WB (Western Blot)
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Rabbit anti-Human SLC10A1 Polyclonal Antibody | anti-SLC10A1 antibody

SLC10A1 Antibody (C-term)

Gene Names
SLC10A1; NTCP
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SLC10A1, Antibody; SLC10A1 Antibody (C-term); Sodium/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1; SLC10A1; NTCP; anti-SLC10A1 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SLC10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-343 amino acids from the C-terminal region of human SLC10A1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
316-343
Sequence Length
349
Applicable Applications for anti-SLC10A1 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Anti-SLC10A1 Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-SLC10A1 Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-SLC10A1 Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-SLC10A1 Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-SLC10A1 Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-SLC10A1 antibody
Sodium/bile acid cotransporters are integral membrane
glycoproteins that participate in the enterohepatic circulation of
bile acids. Two homologous transporters are involved in the
reabsorption of bile acids, one absorbing from the intestinal
lumen, the bile duct, and the kidney with an apical localization
(SLC10A2; MIM 601295), and the other being found in the basolateral
membranes of hepatocytes (SLC10A1).
Product Categories/Family for anti-SLC10A1 antibody
References
Ho, R.H., et al. J. Biol. Chem. 279(8):7213-7222(2004)
Trauner, M., et al. Physiol. Rev. 83(2):633-671(2003)
Hallen, S., et al. Biochemistry 41(23):7253-7266(2002)
Shiao, T., et al. Genomics 69(2):203-213(2000)
Hagenbuch, B., et al. J. Clin. Invest. 93(3):1326-1331(1994)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38119
NCBI Official Full Name
sodium/bile acid cotransporter
NCBI Official Synonym Full Names
solute carrier family 10 (sodium/bile acid cotransporter), member 1
NCBI Official Symbol
SLC10A1
NCBI Official Synonym Symbols
NTCP
NCBI Protein Information
sodium/bile acid cotransporter
UniProt Protein Name
Sodium/bile acid cotransporter
UniProt Gene Name
SLC10A1
UniProt Synonym Gene Names
NTCP
UniProt Entry Name
NTCP_HUMAN

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Product Notes

The SLC10A1 slc10a1 (Catalog #AAA28775) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 316-343. The SLC10A1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC10A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the SLC10A1 slc10a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC10A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.