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product-image-AAA31259_IHC8.jpg IHC (Immunohistochemistry-Paraffin) (AAA31259 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Rabbit SLC1A5 Polyclonal Antibody | anti-SLC1A5 antibody

SLC1A5 Antibody

Gene Names
SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SLC1A5, Antibody; SLC1A5 Antibody; ASCT2; AAAT; AAAT_HUMAN; ATB(0); ATBO; Baboon M7 virus receptor; FLJ31068; M7V1; M7VS1; Neutral amino acid transporter B(0); R16; RD114/simian type D retrovirus receptor; RDR; RDRC; SLC1A5; Sodium dependent neutral amino acid transporter type 2; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 (neutral amino acid transporter); member 5; Solute carrier family 1 member 5; anti-SLC1A5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
SLC1A5 Antibody detects endogenous levels of total SLC1A5.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
541
Applicable Applications for anti-SLC1A5 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human SLC1A5, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry-Paraffin)

(AAA31259 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31259_IHC8.jpg IHC (Immunohistochemistry-Paraffin) (AAA31259 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31259 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31259_IHC7.jpg IHC (Immunohistochemistry-Paraffin) (AAA31259 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin)

(AAA31259 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31259_IHC6.jpg IHC (Immunohistchemistry-Paraffin) (AAA31259 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31259 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31259_IHC5.jpg IHC (Immunohistochemistry-Paraffin) (AAA31259 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31259 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31259_IHC4.jpg IHC (Immunohistochemistry-Paraffin) (AAA31259 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31259 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31259_IHC3.jpg IHC (Immunohistochemistry-Paraffin) (AAA31259 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31259 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31259_IHC2.jpg IHC (Immunohistochemistry-Paraffin) (AAA31259 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from various samples, using SLC1A5 Antibody.Lane 1: Mouse testis, treated with blocking peptide;Lane 2: Mouse testis;Lane 3: Hybridoma cells.Observed bands: 45 kDa.)

product-image-AAA31259_WB.jpg WB (Western Blot) (Western blot analysis of extracts from various samples, using SLC1A5 Antibody.Lane 1: Mouse testis, treated with blocking peptide;Lane 2: Mouse testis;Lane 3: Hybridoma cells.Observed bands: 45 kDa.)
Related Product Information for anti-SLC1A5 antibody
Function: Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids (PubMed:8702519, PubMed:29872227). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904).(Microbial infection) Acts as a cell surface receptor for Feline endogenous virus RD114.(Microbial infection) Acts as a cell surface receptor for Baboon M7 endogenous virus.(Microbial infection) Acts as a cell surface receptor for type D simian retroviruses.

Subcellular Location: Cell membrane>Multi-pass membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity: Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

Subunit Structure: Homotrimer (Probable) (PubMed:29872227). Interacts with ERVH48-1/suppressyn; may negatively regulate syncytialization (PubMed:23492904).

Similarity: Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)56kD, 45kD.
Predicted Molecular Weight: (Calculated)57kDa.
NCBI Official Full Name
neutral amino acid transporter B(0) isoform 1
NCBI Official Synonym Full Names
solute carrier family 1 member 5
NCBI Official Symbol
SLC1A5
NCBI Official Synonym Symbols
R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
NCBI Protein Information
neutral amino acid transporter B(0)
UniProt Protein Name
Neutral amino acid transporter B(0)
UniProt Gene Name
SLC1A5
UniProt Synonym Gene Names
ASCT2; M7V1; RDR; RDRC; ATB(0)
UniProt Entry Name
AAAT_HUMAN

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Product Notes

The SLC1A5 slc1a5 (Catalog #AAA31259) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC1A5 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's SLC1A5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SLC1A5 slc1a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC1A5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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