IF (Immunofluorescence)
(Immunofluorescence staining of A549 cell with AAA18854 at 1:50, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Rabbit anti-Human Sterol O-acyltransferase 1 Polyclonal Antibody | anti-SOAT1 antibody
Rabbit anti-human Sterol O-acyltransferase 1 polyclonal Antibody(SOAT1)
Gene Names
SOAT1; ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purified
Synonyms
Sterol O-acyltransferase 1, Antibody; Rabbit anti-human Sterol O-acyltransferase 1 polyclonal Antibody(SOAT1); Acyl-coenzyme A:cholesterol acyltransferase 1; ACAT-1; Cholesterol acyltransferase 1; SOAT1; ACACT; ACACT1; ACAT; ACAT1; SOAT; STAT; anti-SOAT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid; PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Sequence Length
492
Applicable Applications for anti-SOAT1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:20-1:200
IF: 1:20-1:200
IF: 1:20-1:200
Conjugate
Non-conjugated
Immunogen
Recombinant Human Sterol O-acyltransferase 1 protein (1-130AA)
Immunogen Species
Homo Sapiens (Human)
Research Area
Cardiovascular
Target Names
SOAT1
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of A549 cell with AAA18854 at 1:50, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of A549 cell with AAA18854 at 1:50, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IHC (Immunohistochemistry)
(IHC image of AAA18854 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA18854 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA18854 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissueperformed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA18854 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissueperformed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
sterol O-acyltransferase 1 isoform 2
NCBI Official Synonym Full Names
sterol O-acyltransferase 1
NCBI Official Symbol
SOAT1
NCBI Official Synonym Symbols
ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1
NCBI Protein Information
sterol O-acyltransferase 1
UniProt Protein Name
Sterol O-acyltransferase 1
UniProt Gene Name
SOAT1
UniProt Synonym Gene Names
ACACT; ACACT1; ACAT; ACAT1; SOAT; STAT; ACAT-1
UniProt Entry Name
SOAT1_HUMAN
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Product Notes
The SOAT1 soat1 (Catalog #AAA18854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Sterol O-acyltransferase 1 polyclonal Antibody(SOAT1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sterol O-acyltransferase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:20-1:200 IF: 1:20-1:200. Researchers should empirically determine the suitability of the SOAT1 soat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sterol O-acyltransferase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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