Rabbit TFEB Polyclonal Antibody | anti-TFEB antibody

TFEB Polyclonal Antibody

Cat. Num. AAA28118

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity Purification
• Synonyms: TFEB, Antibody; TFEB Polyclonal Antibody; TFEB; ALPHATFEB; BHLHE35; TCFEB; anti-TFEB antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Sequence Length: 476

• Applicable Applications for anti-TFEB antibody: Western Blot (WB), Immunohistochemistry (IHC-p), Immnofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB 1:500 - 1:2000; IHC-P 1:50 - 1:200; IF/ICC 1:50 - 1:200; ELISA: Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-173 of human TFEB (NP_009093.1)
• Species/Cross-Reactivity: Human, Mouse, Rat
• Preparation and Storage: Store at -20 degrees C. Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of PC-12 cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of TFEB in paraffin-embedded rat testis tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of TFEB in paraffin-embedded mouse lung tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of TFEB in paraffin-embedded human colon tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) HeLa cells using [KD Validated] TFEB Rabbit pAb (AAA28118) at 1:1000 dilution incubated overnight at 4 degrees C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

WB (Western Blot)

(Western blot analysis of various lysates, using TFEB Rabbit pAb (AAA28118) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

Related Product Information for anti-TFEB antibody

Background: Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including cellular response to amino acid starvation; lysosome localization; and positive regulation of autophagy. Located in cytosol; lysosomal membrane; and nucleoplasm.

Product Categories/Family for anti-TFEB antibody

Polyclonal

References

Horibe A, Eid N, Ito Y, et al. Upregulated autophagy in Sertoli cells of ethanol-treated rats is associated with induction of inducible nitric oxide synthase (iNOS), Androgen Receptor Suppression and Germ Cell Apoptosis Int. J. Mol. Sci. 2017, 18(5), 1061; doi: 10.3390/ijms18051061. The TFEB pAb was used for WB, IHC (paraffin) and IF in the publication.

NCBI and Uniprot Product Information

• NCBI GI #: 19856774
• NCBI GeneID: 7942
• UniProt Accession #: P19484; Q709B3; Q7Z6P9; Q9BRJ5; Q9UJD8
• Molecular Weight: Observed MW: 65-70kDa; Calculated MW: 53kDa
• NCBI Official Full Name: Transcription factor EB
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• UniProt Protein Name: Transcription factor EB
• UniProt Gene Name: TFEB
• UniProt Synonym Gene Names: BHLHE35; bHLHe35
• UniProt Entry Name: TFEB_HUMAN