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FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
Rabbit TGF-Beta 1 Polyclonal Antibody | anti-TGFB1 antibody
TGF-Beta 1 Antibody
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Peptide affinity purified
Synonyms
TGF-Beta 1, Antibody; TGF-Beta 1 Antibody; Cartilage-inducing factor antibody; CED antibody; Differentiation inhibiting factor antibody; DPD1 antibody; LAP antibody; Latency-associated peptide antibody; Prepro transforming growth factor beta 1 antibody; TGF beta 1 antibody; TGF beta antibody; TGF beta 1 protein antibody; TGF-beta 1 protein antibody; TGF-beta-1 antibody; TGF-beta-5 antibody; TGF-beta1 antibody; TGFB antibody; Tgfb-1 antibody; tgfb1 antibody; TGFB1_HUMAN antibody; TGFbeta antibody; TGFbeta1 antibody; Transforming Growth Factor b1 antibody; Transforming Growth Factor beta 1 antibody; Transforming growth factor beta 1a antibody; transforming growth factor beta-1 antibody; transforming growth factor; beta 1 antibody; Transforming Growth Factor-?1 antibody; anti-TGFB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Peptide affinity purified
Form/Format
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TGFB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1,000
IHC: 1:200
ICC: 1:200
FC:1:100
IHC: 1:200
ICC: 1:200
FC:1:100
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at 4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in SKBR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in SKBR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control:Lane 1: RajiLane 2: MCF-2Lane 3: A549 Lane 4: Human kidney.)
WB (Western Blot)
(Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control:Lane 1: RajiLane 2: MCF-2Lane 3: A549 Lane 4: Human kidney.)
WB (Western Blot)
(Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control:Lane 1: RajiLane 2: MCF-2Lane 3: A549Lane 4: Human kidney)
WB (Western Blot)
(Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control:Lane 1: RajiLane 2: MCF-2Lane 3: A549Lane 4: Human kidney)
Related Product Information for anti-TGFB1 antibody
Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly.
Product Categories/Family for anti-TGFB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44 kDa
NCBI Official Full Name
transforming growth factor beta-1
NCBI Official Synonym Full Names
transforming growth factor, beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
NCBI Protein Information
transforming growth factor beta-1; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP
UniProt Entry Name
TGFB1_HUMAN
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Product Notes
The TGFB1 tgfb1 (Catalog #AAA29953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TGF-Beta 1 Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's TGF-Beta 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1,000 IHC: 1:200 ICC: 1:200 FC:1:100. Researchers should empirically determine the suitability of the TGFB1 tgfb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF-Beta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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