Rabbit ZC3H12A Polyclonal Antibody | anti-ZC3H12A antibody
ZC3H12A (CT) Antibody
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
The immunogen is located within the last 50 amino acids of ZC3H12A.
IHC (Immunohistochemistry)
(Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistchemistry)
(Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse LiverImmunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin)
IHC (Immunohistochemistry)
(Figure 5. Immunohistochemistry Validation of ZC3H12A in Human TestisImmunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IF (Immunofluorescence)
(Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with AAA11042 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 3. Western Blot Validation in Rat Tissues Loading: 15ug of lysates per lane. Antibodies: ZC3H12A AAA11042, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: ZC3H12A AAA11042, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1. WB Validation in Human Lung Loading: 15ug of lysate per lane. Antibodies: ZC3H12A AAA11042, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2ug/mL Lane 2: 4ug/mL)
Nowak et al. RNA Biol. 2021; 18(1):144-156
Tyka et al. Biochim Biophys Acta Mol Basis Dis. 2021; 1867(10):166199
Boratyn et al. J Cell Biochem. 2020; 121(5-6);3406-3425
NCBI and Uniprot Product Information
Observed: 66 kDa