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5 results for " Notch Proteins" - showing 1-5


IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ITCH antibody at dilution of 1:100 (40x lens).)

ITCH, Polyclonal Antibody (Cat# AAA28315)

Full Name
[KO Validated] ITCH Rabbit pAb
Gene Names
ITCH; AIF4; AIP4; NAPP1; dJ468O1.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
FCM (Flow Cytometry) (T98G cells were stained with AAA14690 (filled histogram) or isotype control (open histogram).)

Delta-like 1, Monoclonal Antibody (Cat# AAA14690)

Full Name
Mouse Anti-Delta-like 1
Reactivity
Human
Applications
FC
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
IP (Immunoprecipitation) (HDAC2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate4 uL anti-HDAC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HDAC2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)

HDAC2, Polyclonal Antibody (Cat# AAA27718)

Full Name
Anti-HDAC2 Antibody, Rabbit Polyclonal
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

Full Name
Anti-Musashi 1/Msi1 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)

DLL3, Polyclonal Antibody (Cat# AAA26850)

Full Name
DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)
Gene Names
DLL3; SCDO1
Reactivity
Human
Applications
IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing

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