Application Data
(The purity of MLL2 (KMT2D) was determined to be >80% by densitometry, approx. MW 51kDa)
MLL2 active protein
MLL2 (KMT2D), Active
Gene Names
KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
Applications
Methyltransferase Assay
Purity
>80%
Synonyms
MLL2; N/A; MLL2 (KMT2D), Active; MLL2 active protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>80%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.05 mg/ml (varies by lot)
Sequence Positions
5317-end
Sequence Length
19432
Applicable Applications for MLL2 active protein
Methyltransferase Assay
Species
Human
Spcific Activity
2.5 nmol/min/mg
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Storage: Store product at -70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships with dry ice.
Stability: 1 year at -70°C from date of shipment.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships with dry ice.
Stability: 1 year at -70°C from date of shipment.
Application Data
(The purity of MLL2 (KMT2D) was determined to be >80% by densitometry, approx. MW 51kDa)
Application Data
(The purity of MLL2 (KMT2D) was determined to be >80% by densitometry, approx. MW 51kDa)
Application Data
(The specific activity of MLL2 (KMT2D) was determined to be 2.5 nmol/min/mg as per activity assay protocol.)
Application Data
(The specific activity of MLL2 (KMT2D) was determined to be 2.5 nmol/min/mg as per activity assay protocol.)
Related Product Information for MLL2 active protein
Description: Recombinant human MLL2 (5317-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Histone-lysine N-methyltransferase 2D, also known as mixed-lineage leukemia 2 (MLL2), methylates 'Lys-4' of histone H3 (H3K4me). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. MLL2/KMT2D is among the most frequently mutated genes in a variety of pediatric tumors.
Scientific Background: Histone-lysine N-methyltransferase 2D, also known as mixed-lineage leukemia 2 (MLL2), methylates 'Lys-4' of histone H3 (H3K4me). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. MLL2/KMT2D is among the most frequently mutated genes in a variety of pediatric tumors.
Product Categories/Family for MLL2 active protein
References
1. Rao RC, et al: Hijacked in cancer: the KMT2 (MLL) family of methyltransferases. Nat Rev Cancer. 15 (6):334-46. 2015. 2. Huether R. et al: The landscape of somatic mutations in epigenetic regulators across 1,000 paediatric cancer genomes. Nat Commun 5: 3630. 2014.
http://www.uniprot.org/uniprot/O14686
http://www.uniprot.org/uniprot/O14686
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51 kDa
NCBI Official Full Name
Homo sapiens lysine methyltransferase 2D (KMT2D), mRNA
NCBI Official Synonym Full Names
lysine methyltransferase 2D
NCBI Official Symbol
KMT2D
NCBI Official Synonym Symbols
ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
NCBI Protein Information
histone-lysine N-methyltransferase 2D
UniProt Protein Name
Histone-lysine N-methyltransferase 2D
UniProt Gene Name
KMT2D
UniProt Synonym Gene Names
ALR; MLL2; MLL4; Lysine N-methyltransferase 2D
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Product Notes
The MLL2 kmt2d (Catalog #AAA73022) is an Active Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 5317-end. AAA Biotech's MLL2 can be used in a range of immunoassay formats including, but not limited to, Methyltransferase Assay. Researchers should empirically determine the suitability of the MLL2 kmt2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLL2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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