SDS-PAGE
(SDS-PAGE of his-tagged human HOP protein (SPR-302).)
HOP Recombinant Protein | STIP1 recombinant protein
Recombinant Human HOP
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Reactivity
Human
Applications
Functional Assay, Western Blot
Synonyms
HOP; N/A; Recombinant Human HOP; Hsp co-chaperone; STIP1 recombinant protein
Host
Human
Reactivity
Human
Form/Format
Affinity Purified using His tag
Concentration
1mg/mL (varies by lot)
Sequence Length
543
Applicable Applications for STIP1 recombinant protein
FA (Functional Assay), WB (Western Blot)
Certificate of Analysis
This product has been certified >90% pure using SDS PAGE analysis. 4uM SPR-302, when added to 2uM SPR-300 (Aha1)-activated Hsp90 (2uM; His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
Conjugate Tag
His tag
Expression System
E Coli
Storage Buffer
20mM HEPES buffer pH7.2, 80mM NaCl, 10% glyercol
Research Area(s)
Chaperones; Heat Shock; Trafficking
Cellular Localization
Cytoplasm, Nucleus
Preparation and Storage
-20 degree C; Avoid freeze/thaw cycle.
SDS-PAGE
(SDS-PAGE of his-tagged human HOP protein (SPR-302).)
SDS-PAGE
(SDS-PAGE of his-tagged human HOP protein (SPR-302).)
Related Product Information for STIP1 recombinant protein
Background Info: Molecular weight of approximately 63kDa
Scientific Background: Hop (Hsp70/Hsp90 Organizing Protein), or Stress-induced Phosphoprotein 1 (STI1) as it is also known, is a 60kDa protein that belongs to the large group of co-chaperones which regulate and assist the major chaperones. It is located in diverse cellular regions and can move between the cytoplasm and the nucleus. It functions to reversibly link together the protein chaperones Hsp70 and Hsp90. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. Hsp70 binding has been localized to TRP1 and sp90 binding have been localized to TPR2a (1). It has also been found to modulate the chaperone activities of the linked proteins and possible interacts with other chaperones and proteins. It has also been found to participate in other complexes besides the Hsp70/Hsp90 one (2). HOP is closely related to human 63kDa protein that is sensitive to simian virus SV40 transformation, and is related to the yeast heat-shock- responsive STI1 gene product (3, 4).
Scientific Background: Hop (Hsp70/Hsp90 Organizing Protein), or Stress-induced Phosphoprotein 1 (STI1) as it is also known, is a 60kDa protein that belongs to the large group of co-chaperones which regulate and assist the major chaperones. It is located in diverse cellular regions and can move between the cytoplasm and the nucleus. It functions to reversibly link together the protein chaperones Hsp70 and Hsp90. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. Hsp70 binding has been localized to TRP1 and sp90 binding have been localized to TPR2a (1). It has also been found to modulate the chaperone activities of the linked proteins and possible interacts with other chaperones and proteins. It has also been found to participate in other complexes besides the Hsp70/Hsp90 one (2). HOP is closely related to human 63kDa protein that is sensitive to simian virus SV40 transformation, and is related to the yeast heat-shock- responsive STI1 gene product (3, 4).
Product Categories/Family for STIP1 recombinant protein
References
1. Flom G., Behal R.H., Rosen L., Cole D.G., Johnson J.L. (2007) Biochem J. 404(1): 159-167. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt3): 789-796. 3. Honore B.H., et al. (1992) J Biol Chem. 267: 8485- 8491. 4. Nicolet C.M., et al. (1989) Mol Cell Bio. 9: 3638-3646.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~63kD
NCBI Official Full Name
stress-induced-phosphoprotein 1 isoform b
NCBI Official Synonym Full Names
stress-induced phosphoprotein 1
NCBI Official Symbol
STIP1
NCBI Official Synonym Symbols
HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
NCBI Protein Information
stress-induced-phosphoprotein 1; NY-REN-11 antigen; Hsp70/Hsp90-organizing protein; hsc70/Hsp90-organizing protein; renal carcinoma antigen NY-REN-11; transformation-sensitive protein IEF SSP 3521; epididymis secretory sperm binding protein Li 94n
UniProt Protein Name
Stress-induced-phosphoprotein 1
UniProt Gene Name
STIP1
UniProt Synonym Gene Names
STI1; Hop
UniProt Entry Name
STIP1_HUMAN
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Product Notes
The STIP1 stip1 (Catalog #AAA103773) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human HOP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOP can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), WB (Western Blot). Researchers should empirically determine the suitability of the STIP1 stip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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