SDS-PAGE
Thrombospondin-1/THBS1/TSP-1 Recombinant Protein | THBS1 recombinant protein
Recombinant Human Thrombospondin-1/THBS1/TSP-1 Protein (C-His)
Gene Names
THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
Purity
> 85% as determined by reducing SDS-PAGE.
Synonyms
Thrombospondin-1/THBS1/TSP-1; N/A; Recombinant Human Thrombospondin-1/THBS1/TSP-1 Protein (C-His); Thrombospondin-1; THBS1; TSP; TSP1; THBS1 recombinant protein
Host
Human Cells
Purity/Purification
> 85% as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Sequence
Asn19-Pro1170
Species
Human
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Tag
C-His
Reconstitution
We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
Preparation and Storage
Storage:
Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
SDS-PAGE
SDS-PAGE
Related Product Information for THBS1 recombinant protein
Protein Construction: Recombinant Human Thrombospondin-1 is produced by our mammalian expression system and the target gene encoding Asn19-Pro1170 is expressed with a 10His tag at the C-terminus.
Background: Thrombospondin-1 (TSP-1) is a 150-180kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1domains, 2 EGF-like domains, and 8 TSP type3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules. TSP-1 counteracts the angiogenic, hypotensive, and antithrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells(EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP).
Background: Thrombospondin-1 (TSP-1) is a 150-180kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1domains, 2 EGF-like domains, and 8 TSP type3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules. TSP-1 counteracts the angiogenic, hypotensive, and antithrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells(EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Mol_Mass: 129.2 kDa
AP_Mol_Mass: 130 & 170 kDa
AP_Mol_Mass: 130 & 170 kDa
NCBI Official Full Name
Thrombospondin-1
NCBI Official Synonym Full Names
thrombospondin 1
NCBI Official Symbol
THBS1
NCBI Official Synonym Symbols
TSP; THBS; TSP1; TSP-1; THBS-1
NCBI Protein Information
thrombospondin-1
UniProt Protein Name
Thrombospondin-1
UniProt Gene Name
THBS1
UniProt Synonym Gene Names
TSP; TSP1
UniProt Entry Name
TSP1_HUMAN
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Product Notes
The THBS1 thbs1 (Catalog #AAA174694) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Asn19-Pro1 170. It is sometimes possible for the material contained within the vial of "Thrombospondin-1/THBS1/TSP-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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