Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC.)
Transmembrane 4 L6 family member 1 (TM4SF1) Recombinant Protein | TM4SF1 recombinant protein
Recombinant Human Transmembrane 4 L6 family member 1 (TM4SF1)-VLPs (Active)
Gene Names
TM4SF1; L6; H-L6; M3S1; TAAL6
Purity
The purity information is not available for VLPs proteins.
Synonyms
Transmembrane 4 L6 family member 1 (TM4SF1); N/A; Recombinant Human Transmembrane 4 L6 family member 1 (TM4SF1)-VLPs (Active); M3S1; TAAL6; TM4SF1 recombinant protein
Host
Mammalian cell
Purity/Purification
The purity information is not available for VLPs proteins.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Sequence Positions
1-202aa; Full Length
Sequence
MCYGKCARCIGHSLVGLALLCIAANILLYFPNGETKYASENHLSRFVWFFSGIVGGGLLMLLPAFVFIGLEQDDCCGCCGHENCGKRCAMLSSVLAALIGIAGSGYCVIVAALGLAEGPLCLDSLGQWNYTFASTEGQYLLDTSTWSECTEPKHIVEWNVSLFSILLALGGIEFILCLIQVINGVLGGICGFCCSHQQQYDC
Species
Homo sapiens (Human)
Tag
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Research Area
CancerLess than 1.0 EU/ug as determined by LAL method.
Transmembrane Domain
4TM
Biological_Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TM4SF1 at 5 ug/mL can bind Anti-TM4SF1 recombinant antibody. The EC50 is 4.079-4.472 ng/mL.The VLPs is negative control.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC.)
Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC.)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human TM4SF1 at 5ug/mL can bind Anti-TM4SF1 recombinant antibody , the EC50 is 4.079-4.472 ng/mL.VLPs is negative control.)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human TM4SF1 at 5ug/mL can bind Anti-TM4SF1 recombinant antibody , the EC50 is 4.079-4.472 ng/mL.VLPs is negative control.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody.The two bands respectively correspond to monomer, Homodimer.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody.The two bands respectively correspond to monomer, Homodimer.)
Product Categories/Family for TM4SF1 recombinant protein
References
"Cloning and expression of the tumor-associated antigen L6."Marken J.S., Schieven G.L., Hellstroem I., Hellstroem K.E., Aruffo A.Proc. Natl. Acad. Sci. U.S.A. 89:3503-3507 (1992)"Membrane topology of the L6 antigen and identification of the protein epitope recognized by the L6 monoclonal antibody."Marken J.S., Bajorath J., Edwards C.P., Farr A.G., Schieven G.L., Hellstroem I., Hellstroem K.E., Aruffo A.J. Biol. Chem. 269:7397-7401 (1994)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,632 Da
NCBI Official Full Name
transmembrane 4 L6 family member 1
NCBI Official Synonym Full Names
transmembrane 4 L six family member 1
NCBI Official Symbol
TM4SF1
NCBI Official Synonym Symbols
L6; H-L6; M3S1; TAAL6
NCBI Protein Information
transmembrane 4 L6 family member 1; tumor-associated antigen L6; transmembrane 4 superfamily member 1; membrane component chromosome 3 surface marker 1; membrane component, chromosome 3, surface marker 1
UniProt Protein Name
Transmembrane 4 L6 family member 1
UniProt Gene Name
TM4SF1
UniProt Synonym Gene Names
M3S1; TAAL6
UniProt Entry Name
T4S1_HUMAN
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Product Notes
The TM4SF1 tm4sf1 (Catalog #AAA279274) is a Recombinant Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-202aa; Full Length. The amino acid sequence is listed below: MCYGKCARCI GHSLVGLALL CIAANILLYF PNGETKYASE NHLSRFVWFF SGIVGGGLLM LLPAFVFIGL EQDDCCGCCG HENCGKRCAM LSSVLAALIG IAGSGYCVIV AALGLAEGPL CLDSLGQWNY TFASTEGQYL LDTSTWSECT EPKHIVEWNV SLFSILLALG GIEFILCLIQ VINGVLGGIC GFCCSHQQQY DC. It is sometimes possible for the material contained within the vial of "Transmembrane 4 L6 family member 1 (TM4SF1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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