SDS-PAGE
Vaccinia Virus B18R/B19R Recombinant Protein | B18R recombinant protein
Vaccinia Virus B18R/B19R Protein (His Tag)
Purity
>90% as determined by SDS-PAGE
Synonyms
Vaccinia Virus B18R/B19R; N/A; Vaccinia Virus B18R/B19R Protein (His Tag); bone marrow stromal cell antigen 2; B18R recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% Glycerol, pH8.0, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
Sequence
Met1-Glu351
Source
Vaccinia Virus
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
His 20
Tag
His Tag
Protein Construction
A DNA sequence encoding the B18R (P25213)(Met1-Glu351) was expressed with a C-terminal polyhistidine tag.
Reconstitution
It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. Recommended that the protein be aliquoted into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for B18R recombinant protein
Background: B18R is a type I interferon (IFN)-binding protein, which is encoded by the B18R open reading frame in the WR (WesternReserve)strain of vaccinia virus. Itis alsoknownasB19RintheCopenhagenstrainofVaccinia. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. It has been shown that secreted B18R binds to uninfected and infected cells. It presents at the cell surface and protects cells from the antiviral state induced by IFN-alpha and IFN-beta. Binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.
References
I Vancov. et al., 1998, J Gen Virol July. 79 (7): 1647-9. Colamonici OR. et al., 1995, J Biol Chem. 270 (27): 15974-8. Goebel SJ. et al., 1990, Virology 179: 247-66. Goebel SJ. et al., 2010, J Cell Biol. 190 (4): 533-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Mass: The secreted recombinant B18R consists of 342 amino acids and predicts a molecular mass of 39.8 KDa. The apparent molecular mass of the protein is approximately 46 KDa in SDS-PAGE under reducing conditions due to glycosylation.
NCBI Official Full Name
IFN-alpha/beta-receptor-like secreted glycoprotein
NCBI Official Symbol
B19R
NCBI Protein Information
IFN-alpha/beta-receptor-like secreted glycoprotein
UniProt Protein Name
Soluble interferon alpha/beta receptor B18
UniProt Gene Name
VACWR200
UniProt Synonym Gene Names
B18
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Product Notes
The B18R vacwr200 (Catalog #AAA258066) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Glu35 1. It is sometimes possible for the material contained within the vial of "Vaccinia Virus B18R/B19R, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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