Human 2, 3-Disphosphoglycerate, 2, 3-DPG ELISA Kit | 2, 3DPG elisa kit
Human 2, 3-Disphosphoglycerate, 2, 3-DPG ELISA Kit
Reactivity
Human
Synonyms
2, 3-Disphosphoglycerate, 2, 3-DPG; N/A; Human 2, 3-Disphosphoglycerate, 2, 3-DPG ELISA Kit; Human 2; 3-Disphosphoglycerate; 2; 3-DPG ELISA Kit; 3-DPG; 2, 3DPG elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human 2, 3-DPG. No significant cross-reactivity or interference between human 2, 3-DPG and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
0.312 umol/ml-20 umol/ml
Sensitivity
< 0.078 umol/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for 2, 3DPG elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 2, 3-DPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 2, 3-DPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 2, 3-DPG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 2, 3-DPG bound in the initial step. The color development is stopped and the intensity of the color is measured.
