Colony Stimulating Factor 1, Macrophage (MCSF) Active Protein | MCSF active protein

Active Colony Stimulating Factor 1, Macrophage (MCSF)

Cat. Num. AAA150971

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Applications: Activity Assay, Cell Culture
• Purity: Greater than 95% by SDS-PAGE
• Synonyms: Colony Stimulating Factor 1, Macrophage (MCSF); N/A; Active Colony Stimulating Factor 1, Macrophage (MCSF); MCSF/Colony Stimulating Factor 1, Macrophage; CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1; MCSF active protein

• Host: 293F Cell
• Purity/Purification: Greater than 95% by SDS-PAGE
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence: Lys33~Glu262

• Applicable Applications for MCSF active protein: Activity Assay, Cell Culture
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Tag: N-terminal His Tag
• Biological Activity: Active
• Expression System: Eukaryotic expression
• Organism Species: Mouse
• Isoelectric Point: 5.1
• Subcellular Location: Secreted
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

Sequence

Bioactivity

(The binding activity of M-CSF with M-CSF-R.)

SDS-PAGE

(SDS-PAGE)

Sequence

(Gene Sequencing (extract))

Related Product Information for MCSF active protein

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is asecreted cytokine which influences hematopoietic stem cells to differentiateinto macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoieticgrowth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affectsmacrophages and monocytes in several ways, including stimulating increasedphagocytic and chemotactic activity, and increased tumour cell cytotoxicity. Therole of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF-1R or M-CSF-R encoded by thec-fms proto-oncogene), M-CSF also modulates the proliferation of earlierhematopoietic progenitors and influence numerous physiological processesinvolved in immunology, metabolism, fertility and pregnancy. Besides, ColonyStimulating Factor Receptor, Macrophage (M-CSF-R) has been identified as aninteractor of M-CSF, thus a binding ELISA assay was conducted to detect theinteraction of recombinant mouse M-CSF and recombinant mouse M-CSF-R. Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100uL were then transferred to M-CSF-R coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRPlabelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of M-CSF and M-CSF-R was shown in Figure 1, and this effect was in adose dependent manner.

Product Categories/Family for MCSF active protein

Cytokine; Infection immunity; Active, eukaryotic recombinant

NCBI and Uniprot Product Information

• NCBI GI #: 166064046
• NCBI GeneID: 12977
• NCBI Accession #: NP_001107001.1
• NCBI GenBank Nucleotide #: NM_001113529.1
• UniProt Accession #: P07141; Q3U395; Q8R3C8
• Molecular Weight: 29,353 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform 2
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1; osteopetrosis
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_MOUSE