Bioactivity
(GLB1 is a lysosomal beta -galactosidase that hydrolyzes the terminal beta -galactose from ganglioside and keratan sulfate. Defects in this gene are the causes of lysosomal storage diseases for GM1-gangliosidosis and Morquio B syndrome (also known as mucopolysaccharidosis IVB). In GM1 gangliosidosis, GM1 ganglioside accumulates in the neurons of the central nervous system, because of the deficiency of lysosomal beta -galactosidase activity. GM1 gangliosidosis demonstrates varying degrees of clinical severity but is invariably fatal, and children with the most common and severe form of GM1 gangliosidosis usually die within 3 years of birth. Morquio B syndrome patients are neurologically normal, but display severe skeletal dysostosis multiplex because of an accumulation of keratan sulfate. The activity assay of GLB1 was measured by its ability to cleave a peptide substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The reaction was performed in 50 mM Sodium Citrate, pH 3.5 (Assay Buffer), ainitiated by addition 50 uL of 1.5 ug/ml uPA (diluted by Assay Buffer) to 50 uL of 1.2 mM Substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse GLB1 is >17000 pmol/min/ug.)
Galactosidase Beta (GLb) Active Protein | GLb active protein
Active Galactosidase Beta (GLb)
Gene Names
Glb1; Bge; Bgl; Bgs; Bgt; Bgl-e; Bgl-s; Bgl-t; AW125515; C130097A14Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Galactosidase Beta (GLb); N/A; Active Galactosidase Beta (GLb); Galactosidase Beta; GLb active protein (eukaryotic,293F cell), Mouse; GL-B; b-GAL; bGAL; ELNR1; EBP; GLB1; Lactase; Elastin Receptor 1,67kDa; Elastin Receptor 1(67kD); Acid Beta-Galactosidase4; GLb active protein
Host
293F cell
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Ile25~Ser647
Applicable Applications for GLb active protein
Activity Assay, Cell Culture
Species
Mouse
Expression System
Eukaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
7.3
Research Area
Enzyme & Kinase, Metabolic pathway
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(GLB1 is a lysosomal beta -galactosidase that hydrolyzes the terminal beta -galactose from ganglioside and keratan sulfate. Defects in this gene are the causes of lysosomal storage diseases for GM1-gangliosidosis and Morquio B syndrome (also known as mucopolysaccharidosis IVB). In GM1 gangliosidosis, GM1 ganglioside accumulates in the neurons of the central nervous system, because of the deficiency of lysosomal beta -galactosidase activity. GM1 gangliosidosis demonstrates varying degrees of clinical severity but is invariably fatal, and children with the most common and severe form of GM1 gangliosidosis usually die within 3 years of birth. Morquio B syndrome patients are neurologically normal, but display severe skeletal dysostosis multiplex because of an accumulation of keratan sulfate. The activity assay of GLB1 was measured by its ability to cleave a peptide substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The reaction was performed in 50 mM Sodium Citrate, pH 3.5 (Assay Buffer), ainitiated by addition 50 uL of 1.5 ug/ml uPA (diluted by Assay Buffer) to 50 uL of 1.2 mM Substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse GLB1 is >17000 pmol/min/ug.)
Bioactivity
(GLB1 is a lysosomal beta -galactosidase that hydrolyzes the terminal beta -galactose from ganglioside and keratan sulfate. Defects in this gene are the causes of lysosomal storage diseases for GM1-gangliosidosis and Morquio B syndrome (also known as mucopolysaccharidosis IVB). In GM1 gangliosidosis, GM1 ganglioside accumulates in the neurons of the central nervous system, because of the deficiency of lysosomal beta -galactosidase activity. GM1 gangliosidosis demonstrates varying degrees of clinical severity but is invariably fatal, and children with the most common and severe form of GM1 gangliosidosis usually die within 3 years of birth. Morquio B syndrome patients are neurologically normal, but display severe skeletal dysostosis multiplex because of an accumulation of keratan sulfate. The activity assay of GLB1 was measured by its ability to cleave a peptide substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The reaction was performed in 50 mM Sodium Citrate, pH 3.5 (Assay Buffer), ainitiated by addition 50 uL of 1.5 ug/ml uPA (diluted by Assay Buffer) to 50 uL of 1.2 mM Substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse GLB1 is >17000 pmol/min/ug.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for GLb active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,121 Da
NCBI Official Full Name
beta-galactosidase preproprotein
NCBI Official Synonym Full Names
galactosidase, beta 1
NCBI Official Symbol
Glb1
NCBI Official Synonym Symbols
Bge; Bgl; Bgs; Bgt; Bgl-e; Bgl-s; Bgl-t; AW125515; C130097A14Rik
NCBI Protein Information
beta-galactosidase
UniProt Protein Name
Beta-galactosidase
UniProt Gene Name
Glb1
UniProt Synonym Gene Names
Bgl; Glb-1; Lactase
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Product Notes
The GLb glb1 (Catalog #AAA161714) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Galactosidase Beta (GLb) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the GLb glb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ile25~Ser6 47. It is sometimes possible for the material contained within the vial of "Galactosidase Beta (GLb), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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