Bioactivity
(Hyaluronidase (HAase) is a general term for enzymes that can hydrolyze hyaluronic acid. It is an enzyme that can reduce the activity of hyaluronic acid in the body, thereby improving the fluid permeability in tissues. When used in the human body, it can temporarily reduce the viscosity of the intercellular matrix, promote subcutaneous infusion, locally accumulated exudate or blood to accelerate diffusion and facilitate absorption, and is an important drug dispersant. Clinically used as a drug penetrating agent to promote drug absorption, promote local edema or hematoma dissipation after surgery and trauma. Human HAase consists of 435 amino acids which contains a signal peptide of 1-21 amino acids and it shares 74% and 75% amino acid sequence homology with mouse and rat respectively. The activity assay of recombinant human HAase was measured by its ability to hydrolyze the substrate hyaluronic acid. The rhHAase was diluted to 0.5 ug/ml in 8.46 mM NaH2PO4, 11.54 mM Na2HPO4, 77 mM NaCl, 0.1 mg/ml BSA, pH 7. 50 ul 0.5 ug/ml rhHAase was added into the microplate and start the reaction by adding 50 uL of 0.3 mg/ml substrate which was diluted in 300mM NaH2PO4, pH 5.35. Incubated at 37 degree C for 5min and add 50 ul reaction mixture to 250 ul 1 mg/ml BSA in 24 mM sodium acetic, 79 mM acetic acid, pH 3.75. Incubated at room temperature for 10min and read at a wavelength of 600 nm. The specific activity of recombinant human HAase is >30 pmol/min/ug.)
Hyaluronidase (HAase) Active Protein | HAase active protein
Active Hyaluronidase (HAase)
Gene Names
HYAL1; NAT6; LUCA1; HYAL-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Hyaluronidase (HAase); N/A; Active Hyaluronidase (HAase); Hyaluronidase; HAase active protein (eukaryotic,293F cell), Human; Hyaluronic Lase; Hyaluroniclase4; HAase active protein
Host
293F cell
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Phe22~Trp435
Applicable Applications for HAase active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Eukaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.9
Research Area
Enzyme & Kinase, Tumor immunity, Hepatology
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Hyaluronidase (HAase) is a general term for enzymes that can hydrolyze hyaluronic acid. It is an enzyme that can reduce the activity of hyaluronic acid in the body, thereby improving the fluid permeability in tissues. When used in the human body, it can temporarily reduce the viscosity of the intercellular matrix, promote subcutaneous infusion, locally accumulated exudate or blood to accelerate diffusion and facilitate absorption, and is an important drug dispersant. Clinically used as a drug penetrating agent to promote drug absorption, promote local edema or hematoma dissipation after surgery and trauma. Human HAase consists of 435 amino acids which contains a signal peptide of 1-21 amino acids and it shares 74% and 75% amino acid sequence homology with mouse and rat respectively. The activity assay of recombinant human HAase was measured by its ability to hydrolyze the substrate hyaluronic acid. The rhHAase was diluted to 0.5 ug/ml in 8.46 mM NaH2PO4, 11.54 mM Na2HPO4, 77 mM NaCl, 0.1 mg/ml BSA, pH 7. 50 ul 0.5 ug/ml rhHAase was added into the microplate and start the reaction by adding 50 uL of 0.3 mg/ml substrate which was diluted in 300mM NaH2PO4, pH 5.35. Incubated at 37 degree C for 5min and add 50 ul reaction mixture to 250 ul 1 mg/ml BSA in 24 mM sodium acetic, 79 mM acetic acid, pH 3.75. Incubated at room temperature for 10min and read at a wavelength of 600 nm. The specific activity of recombinant human HAase is >30 pmol/min/ug.)
Bioactivity
(Hyaluronidase (HAase) is a general term for enzymes that can hydrolyze hyaluronic acid. It is an enzyme that can reduce the activity of hyaluronic acid in the body, thereby improving the fluid permeability in tissues. When used in the human body, it can temporarily reduce the viscosity of the intercellular matrix, promote subcutaneous infusion, locally accumulated exudate or blood to accelerate diffusion and facilitate absorption, and is an important drug dispersant. Clinically used as a drug penetrating agent to promote drug absorption, promote local edema or hematoma dissipation after surgery and trauma. Human HAase consists of 435 amino acids which contains a signal peptide of 1-21 amino acids and it shares 74% and 75% amino acid sequence homology with mouse and rat respectively. The activity assay of recombinant human HAase was measured by its ability to hydrolyze the substrate hyaluronic acid. The rhHAase was diluted to 0.5 ug/ml in 8.46 mM NaH2PO4, 11.54 mM Na2HPO4, 77 mM NaCl, 0.1 mg/ml BSA, pH 7. 50 ul 0.5 ug/ml rhHAase was added into the microplate and start the reaction by adding 50 uL of 0.3 mg/ml substrate which was diluted in 300mM NaH2PO4, pH 5.35. Incubated at 37 degree C for 5min and add 50 ul reaction mixture to 250 ul 1 mg/ml BSA in 24 mM sodium acetic, 79 mM acetic acid, pH 3.75. Incubated at room temperature for 10min and read at a wavelength of 600 nm. The specific activity of recombinant human HAase is >30 pmol/min/ug.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for HAase active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
37,439 Da
NCBI Official Full Name
Hyaluronidase-1
NCBI Official Synonym Full Names
hyaluronoglucosaminidase 1
NCBI Official Symbol
HYAL1
NCBI Official Synonym Symbols
NAT6; LUCA1; HYAL-1
NCBI Protein Information
hyaluronidase-1; luCa-1; plasma hyaluronidase; tumor suppressor LUCA-1; lung carcinoma protein 1
UniProt Protein Name
Hyaluronidase-1
UniProt Gene Name
HYAL1
UniProt Synonym Gene Names
LUCA1; Hyal-1; LuCa-1
UniProt Entry Name
HYAL1_HUMAN
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Product Notes
The HAase hyal1 (Catalog #AAA161835) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hyaluronidase (HAase) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the HAase hyal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Phe22~Trp4 35. It is sometimes possible for the material contained within the vial of "Hyaluronidase (HAase), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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