Bioactivity
(IFNgamma R2 (Interferon gamma receptor 2; also called IFN gamma R beta IFN gamma RII, or AF1) is a 6064 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecule). It is highly expressed on the surfaces of myeloid cells, moderately expressed on B cells, and poorly expressed on the surfaces of T cells. IFNgammaR2 is a cell-surface receptor that is required for interferon-gamma signalling and therefore plays a critical immunoregulatory role in innate and adaptive immunity against viral and also bacterial and protozoal infections. IFNgR2 have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFNgamma, the complex contains two each of IFNgamma R1, R2 and Jak1 molecules. Binding of IFNgamma to IFNgamma R1 recruits Jak2 to IFN-gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis. A functional binding ELISA assay was conducted to detect the interaction of recombinant human IFNgR2 and recombinant rat JAK2. Briefly, biotin-linked IFNgR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of IFNgR2 and JAK2 was shown in Figure 1, the EC50 for this effect is 1.02 ug/mL.)
Interferon Gamma Receptor 2 (IFNgR2) Active Protein | IFNgR2 active protein
Active Interferon Gamma Receptor 2 (IFNgR2)
Gene Names
IFNGR2; AF-1; IFGR2; IFNGT1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Interferon Gamma Receptor 2 (IFNgR2); N/A; Active Interferon Gamma Receptor 2 (IFNgR2); Interferon Gamma Receptor 2; IFNgR2 active protein (eukaryotic,293F cell), Human; IFNGT1; AF-1; Interferon Gamma Transducer 1; Interferon gamma receptor accessory factor 14; IFNgR2 active protein
Host
293F cell
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Met1~Gln247
Applicable Applications for IFNgR2 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Eukaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.7
Research Area
CD & Adhesion molecule
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(IFNgamma R2 (Interferon gamma receptor 2; also called IFN gamma R beta IFN gamma RII, or AF1) is a 6064 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecule). It is highly expressed on the surfaces of myeloid cells, moderately expressed on B cells, and poorly expressed on the surfaces of T cells. IFNgammaR2 is a cell-surface receptor that is required for interferon-gamma signalling and therefore plays a critical immunoregulatory role in innate and adaptive immunity against viral and also bacterial and protozoal infections. IFNgR2 have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFNgamma, the complex contains two each of IFNgamma R1, R2 and Jak1 molecules. Binding of IFNgamma to IFNgamma R1 recruits Jak2 to IFN-gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis. A functional binding ELISA assay was conducted to detect the interaction of recombinant human IFNgR2 and recombinant rat JAK2. Briefly, biotin-linked IFNgR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of IFNgR2 and JAK2 was shown in Figure 1, the EC50 for this effect is 1.02 ug/mL.)
Bioactivity
(IFNgamma R2 (Interferon gamma receptor 2; also called IFN gamma R beta IFN gamma RII, or AF1) is a 6064 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecule). It is highly expressed on the surfaces of myeloid cells, moderately expressed on B cells, and poorly expressed on the surfaces of T cells. IFNgammaR2 is a cell-surface receptor that is required for interferon-gamma signalling and therefore plays a critical immunoregulatory role in innate and adaptive immunity against viral and also bacterial and protozoal infections. IFNgR2 have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFNgamma, the complex contains two each of IFNgamma R1, R2 and Jak1 molecules. Binding of IFNgamma to IFNgamma R1 recruits Jak2 to IFN-gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis. A functional binding ELISA assay was conducted to detect the interaction of recombinant human IFNgR2 and recombinant rat JAK2. Briefly, biotin-linked IFNgR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of IFNgR2 and JAK2 was shown in Figure 1, the EC50 for this effect is 1.02 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for IFNgR2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,806 Da
NCBI Official Full Name
interferon gamma receptor 2
NCBI Official Synonym Full Names
interferon gamma receptor 2 (interferon gamma transducer 1)
NCBI Official Symbol
IFNGR2
NCBI Official Synonym Symbols
AF-1; IFGR2; IFNGT1
NCBI Protein Information
interferon gamma receptor 2; IFN-gamma-R2; IFN-gamma receptor 2; interferon gamma transducer 1; interferon gamma receptor beta chain; interferon gamma receptor accessory factor 1; interferon gamma receptor accessory factor-1
UniProt Protein Name
Interferon gamma receptor 2
UniProt Gene Name
IFNGR2
UniProt Synonym Gene Names
IFNGT1; IFN-gamma receptor 2; IFN-gamma-R2; AF-1
UniProt Entry Name
INGR2_HUMAN
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Product Notes
The IFNgR2 ifngr2 (Catalog #AAA161936) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interferon Gamma Receptor 2 (IFNgR2) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the IFNgR2 ifngr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Gln24 7. It is sometimes possible for the material contained within the vial of "Interferon Gamma Receptor 2 (IFNgR2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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