Macrophage Inflammatory Protein 1 Alpha (MIP1a) Active Protein | MIP1a active protein

Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)

Cat. Num. AAA146611

• Gene Names: CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• Applications: Activity Assay, Cell Culture
• Purity: > 95%
• Synonyms: Macrophage Inflammatory Protein 1 Alpha (MIP1a); N/A; Active Macrophage Inflammatory Protein 1 Alpha (MIP1a); CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein; MIP1a active protein

• Host: 293F cell
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 92

• Applicable Applications for MIP1a active protein: Activity Assay, Cell Culture
• Fragment: Ser24~Ala92
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Human
• Isoelectric Point: 4.8
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

Application Data

SDS-PAGE

Activity

(MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers (100ul cell suspension, 106 cells/ml in RPMI 1640 with 0.5% FBS) and MIP-1a (100ng/mL, diluted in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).By counting migrated cells in low chamber at high magnification (×400) randomly, it was shown that a mean of 41.2 THP1 cells/field migrated towards serum free RPMI 1640 medium with 100ng/mL MIP-1a, while only 3.6 THP1 cells/field migrated towards serum free RPMI 1640 medium. And the migrated THP1 cells in low chamber at low magnification (×40) was shown in Figure 1.)

Product Categories/Family for MIP1a active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4506843
• NCBI GeneID: 6348
• NCBI Accession #: NP_002974.1
• NCBI GenBank Nucleotide #: NM_002983.2
• UniProt Accession #: P10147
• Molecular Weight: 9.3kDa; 14kDa
• NCBI Official Full Name: C-C motif chemokine 3
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 3
• NCBI Official Symbol: CCL3
• NCBI Official Synonym Symbols: MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• NCBI Protein Information: C-C motif chemokine 3
• UniProt Protein Name: C-C motif chemokine 3
• UniProt Gene Name: CCL3
• UniProt Synonym Gene Names: G0S19-1; MIP1A; SCYA3; MIP-1-alpha