Bioactivity
(SP-D (surfactant protein-D) is a 43 kDa member of the collectin family of innate immune modulators. It is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that further places it in a subset of an expanded group of proteins termed defense collagens. SP-D is known to bind both SIRP alpha and the calreticulin/CD91 complex on macrophages. When the ratio of antigen/pathogen to available CRDs is low, antigen can be bound without occupying all available CRDs. The free CRDs will bind to SIRP alpha, generating a signal that downmodulates the inflammatory response. When virtually all CRDs are occupied by ligand, however, free CRDs are not available for SIRP alpha binding. The activity of the recombinant human SPD was measured by its ability to bind fluorescein-conjugated E. coli bioparticles. The rhSPD was diluted to 10 ug/ml in assay buffer of 20 mM Tris, 137 mM NaCl, 1 mM CaC12, pH7.4 and fluorescein-conjugated E. coli was diluted to 7*108 cells/ml. Equal volume of 10 ug/ml rhSPD and fluorescein-conjugated E. coli were mixed and incubated at room temperature for 1h. 10 ul mixture was took onto the slide and observed under the fluorescence microscopy. The result was shown in figure 1, the rhSPD could bind fluorescein-conjugated E. coli bioparticles.)
Surfactant Associated Protein D (SPD) Active Protein | SPD active protein
Active Surfactant Associated Protein D (SPD)
Gene Names
SFTPD; SP-D; PSP-D; SFTP4; COLEC7
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Surfactant Associated Protein D (SPD); N/A; Active Surfactant Associated Protein D (SPD); Surfactant Associated Protein D; SPD active protein (eukaryotic,293F cell), Human; SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D4; SPD active protein
Host
293F cell
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Ala21~Phe375
Applicable Applications for SPD active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Eukaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.5
Research Area
Infection immunity, Pulmonology
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(SP-D (surfactant protein-D) is a 43 kDa member of the collectin family of innate immune modulators. It is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that further places it in a subset of an expanded group of proteins termed defense collagens. SP-D is known to bind both SIRP alpha and the calreticulin/CD91 complex on macrophages. When the ratio of antigen/pathogen to available CRDs is low, antigen can be bound without occupying all available CRDs. The free CRDs will bind to SIRP alpha, generating a signal that downmodulates the inflammatory response. When virtually all CRDs are occupied by ligand, however, free CRDs are not available for SIRP alpha binding. The activity of the recombinant human SPD was measured by its ability to bind fluorescein-conjugated E. coli bioparticles. The rhSPD was diluted to 10 ug/ml in assay buffer of 20 mM Tris, 137 mM NaCl, 1 mM CaC12, pH7.4 and fluorescein-conjugated E. coli was diluted to 7*108 cells/ml. Equal volume of 10 ug/ml rhSPD and fluorescein-conjugated E. coli were mixed and incubated at room temperature for 1h. 10 ul mixture was took onto the slide and observed under the fluorescence microscopy. The result was shown in figure 1, the rhSPD could bind fluorescein-conjugated E. coli bioparticles.)
Bioactivity
(SP-D (surfactant protein-D) is a 43 kDa member of the collectin family of innate immune modulators. It is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that further places it in a subset of an expanded group of proteins termed defense collagens. SP-D is known to bind both SIRP alpha and the calreticulin/CD91 complex on macrophages. When the ratio of antigen/pathogen to available CRDs is low, antigen can be bound without occupying all available CRDs. The free CRDs will bind to SIRP alpha, generating a signal that downmodulates the inflammatory response. When virtually all CRDs are occupied by ligand, however, free CRDs are not available for SIRP alpha binding. The activity of the recombinant human SPD was measured by its ability to bind fluorescein-conjugated E. coli bioparticles. The rhSPD was diluted to 10 ug/ml in assay buffer of 20 mM Tris, 137 mM NaCl, 1 mM CaC12, pH7.4 and fluorescein-conjugated E. coli was diluted to 7*108 cells/ml. Equal volume of 10 ug/ml rhSPD and fluorescein-conjugated E. coli were mixed and incubated at room temperature for 1h. 10 ul mixture was took onto the slide and observed under the fluorescence microscopy. The result was shown in figure 1, the rhSPD could bind fluorescein-conjugated E. coli bioparticles.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for SPD active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,728 Da
NCBI Official Full Name
pulmonary surfactant-associated protein D
NCBI Official Synonym Full Names
surfactant protein D
NCBI Official Symbol
SFTPD
NCBI Official Synonym Symbols
SP-D; PSP-D; SFTP4; COLEC7
NCBI Protein Information
pulmonary surfactant-associated protein D; collectin 7; collectin-7; lung surfactant protein D; pulmonary surfactant apoprotein; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4
UniProt Protein Name
Pulmonary surfactant-associated protein D
UniProt Gene Name
SFTPD
UniProt Synonym Gene Names
COLEC7; PSPD; SFTP4; PSP-D; SP-D
UniProt Entry Name
SFTPD_HUMAN
Similar Products
Product Notes
The SPD sftpd (Catalog #AAA161827) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Surfactant Associated Protein D (SPD) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the SPD sftpd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala21~Phe3 75. It is sometimes possible for the material contained within the vial of "Surfactant Associated Protein D (SPD), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.