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WB (Western Blot)
(Western BlotSample: Recombinant IL1b, HumanAntibody: Rabbit Anti-Human IL1b Ab)
Interleukin 1 Beta (IL1b) Active Protein | IL1b active protein
Active Interleukin 1 Beta (IL1b)
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Homo sapiens (Human)
Applications
Cell culture ; Activity Assays; In vivo assays.
Purity
>95%
Synonyms
Interleukin 1 Beta (IL1b); N/A; Active Interleukin 1 Beta (IL1b); IL1-B; IL1-Beta; IL1F2; IL-1beta; Interleukin-1 Family Member 2; Catabolin; IL1b active protein
Host
293F cell
Reactivity
Homo sapiens (Human)
Purity/Purification
>95%
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 5% Trehalose
Concentration
200ug/mL (varies by lot)
Applicable Applications for IL1b active protein
Cell culture ; Activity Assays; In vivo assays.
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Eukaryotic expression.
Residues
Ala117~Ser269
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
5.9
Reconstitution
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not
vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
WB (Western Blot)
(Western BlotSample: Recombinant IL1b, HumanAntibody: Rabbit Anti-Human IL1b Ab)
WB (Western Blot)
(Western BlotSample: Recombinant IL1b, HumanAntibody: Rabbit Anti-Human IL1b Ab)
SDS-PAGE
(Sample: Active recombinant IL1b, Human)
SDS-PAGE
(Sample: Active recombinant IL1b, Human)
Gene Sequencing (extract)
Gene Sequencing (extract)
Application Data
Application Data
Activity
(Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-β for 4h and IL-6 levels in the cell supernatant were determined by ELISA.Result: IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-β, the data was shown in Table 1 and Figure 1.)
Activity
(Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-β for 4h and IL-6 levels in the cell supernatant were determined by ELISA.Result: IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-β, the data was shown in Table 1 and Figure 1.)
Sequence Information
Sequence Information
Product Categories/Family for IL1b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 19.0kDa
Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1 beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
NCBI Protein Information
interleukin-1 beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
Similar Products
Product Notes
The IL1b il1b (Catalog #AAA21056) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. The Active Interleukin 1 Beta (IL1b) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 1 Beta (IL1b) can be used in a range of immunoassay formats including, but not limited to, Cell culture ; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the IL1b il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 1 Beta (IL1b), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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