Standard Curve (Sample)
Human Interleukin-1 beta ELISA Kit | IL1B elisa kit
Human Interleukin-1 beta ELISA Kit
Standard Curve (Sample)
Standard Curve (Sample)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Product Notes
The Human IL1B il1b (Catalog #AAA23262) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA23262 ELISA Kit recognizes Human IL1B. It is sometimes possible for the material contained within the vial of "Interleukin-1 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What sample types are compatible with the IL-1β ELISA kit?
The IL-1β ELISA kit is compatible with serum, plasma, cell culture supernatants, cell lysates, and other biological fluids like urine. Some kits also support tissue homogenates.
What is the detection sensitivity and range of the assay?
Sensitivity typically ranges from 0.39–4.69 pg/mL depending on the kit variant, with detection ranges spanning 0.63–1,000 pg/mL. Assay range and sensitivity vary by species and manufacturer formulation.
How long does the IL-1β ELISA protocol take to complete?
Most IL-1β ELISA protocols require 3–4 hours total assay time. This includes incubation periods, washing steps, and substrate development.
What controls are required for accurate IL-1β measurement?
Blank controls, positive controls (reconstituted standard proteins), and negative controls should be included in each assay run. Proper positive and negative controls ensure accurate sample quantification and validate assay performance.
What plate reader settings are needed for detection?
Set the microplate reader to measure absorbance at 450 nm wavelength using a colorimetric detection method. Reference wavelength is typically 655 nm, with absorbance recorded as optical density (OD).
How should the kit be stored to maintain stability?
Store the unopened IL-1β ELISA kit at 2–8°C for up to 6 months or –20°C for up to 1 year. Avoid repeated freeze-thaw cycles. Extended storage at –80°C is recommended for kits requiring longer stability.
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