Rat 3alpha-Androstanediol ELISA Kit | 3alpha-diol elisa kit
Rat 3alpha-Androstanediol ELISA Kit
Reactivity
Rat
Synonyms
3alpha-Androstanediol; N/A; Rat 3alpha-Androstanediol ELISA Kit; Rat 3a-Androstanediol ELISA Kit; 3alpha-diol elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25 ng/mL
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for 3alpha-diol elisa kit
Intended Uses: This 3alphaAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat 3alphaAS. This ELISA kit for research use only!
Principle of the Assay||3alphaAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-3alphaAS antibody and an 3alphaAS-HRP conjugate. The assay sample and buffer are incubated together with 3alphaAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 3alphaAS concentration since 3alphaAS from samples and 3alphaAS-HRP conjugate compete for the anti-3alphaAS antibody binding site. Since the number of sites is limited, as more sites are occupied by 3alphaAS from the sample, fewer sites are left to bind 3alphaAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 3alphaAS concentration in each sample is interpolated from this standard curve.
Principle of the Assay||3alphaAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-3alphaAS antibody and an 3alphaAS-HRP conjugate. The assay sample and buffer are incubated together with 3alphaAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 3alphaAS concentration since 3alphaAS from samples and 3alphaAS-HRP conjugate compete for the anti-3alphaAS antibody binding site. Since the number of sites is limited, as more sites are occupied by 3alphaAS from the sample, fewer sites are left to bind 3alphaAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 3alphaAS concentration in each sample is interpolated from this standard curve.
